CELL BLOCK

Question 1

CELL BLOCK STEPS

Answer 1

Salvaging of grossly visible flecks of tissue or sediment

             Fixation by formalin or any other fixative

                                 Paraffin embedding

                                     Cutting

                                     Staining

Question 2

ADVANTAGES OF CELL BOCK

Answer 2

Slides readily interpretable

Availability of a block facilitates more sections.

Important residual material is salvaged.

Concentrated in a small area of the slide so examination less time consuming

Question 3

SPECIMEN

Answer 3

FNA material

Sputum

Effusion fluids

Urine sediment

Various washings and lavages

Question 4

Methods of cell block preparation

Answer 4

Direct processing of tissue fragments present in fluids

Fixed sediment method

Bacterial agar method

Simplified cell block technique using alcohol, acetone and paraffin

Compact cell block technique

Plasma thrombin method

Cell blocks from millipore

Question 5

Cell block

Answer 5

Adding plasma and thrombin solution
Wrapped in filter paper
Placed in a cassette
Embedded in paraffin
Cut and H&E stain

Question 6

Cytocentrifuge preparation

Answer 6

Alcohol-fixed

Papanicolaou-stained

Question 7

Air-dried cytocentrifuge preparation

Answer 7

Hematologic malignancy is suspected