Cell fractionation

Question 1

Cell fractionation

Answer 1

1. Cell homologised to break open cell
   2.Filter to remove (large) debris

Question 2

Ultracentrifuation

Answer 2

-Centrifuge at low speed to sperate heavy organells (nuclei)
-Respin a higher speed to get mitrochondria in pellet
-Continue to get next largest organele
-Organelles which go to the bottom form a pellet

Question 3

Conditions for cell fractionation

Answer 3

Ice cold- to reduce enzyme activity, so organelles don’t get hydrolysed

Buffered- prevent PH change, to prevent proteins denaturing

Isotonic- prevent osmosis so organelles don’t burst or shrivel