Cell Fractionstion

Question 1

Stage on of cell fractionation

Answer 1

Chop up the tissue (eg. Liver) in an ice cold buffer solution.

Question 2

Step two of cell fractionation

Answer 2

Put the chopped tissue into a blender or homogeniser which breaks open the cells

Question 3

Step 3 of cell fractionation

Answer 3

Filter the mixture to remove the debris

Question 4

Step 4 of cell fractionation

Answer 4

Pour the mixture into tubes and spin very quickly in a centrifuge.

Question 5

How does a centrifuge work

Answer 5

The denser parts of the mixture get spun to the bottom of the tube where they form s ‘pellet’ called the sediment

Question 6

Step 5 of cell fractionation

Answer 6

The liquid layer on top ( the super Nat ant) is poured into a fresh tube,leaving the sediment behind. This contains nuclei.

Question 7

Step 6 of cell fractionation

Answer 7

This supernatant may be spun again faster to produce a sediment containing mitochondria.

Question 8

Step 7 of cell fractionation

Answer 8

The supernatant can be spun again and the sediment left after will contain ribosome,membranes,etc.

Question 9

What does the sediment contain the first time it is spun

Answer 9

It contains nuclei

Question 10

What does the sediment contain the second time it is spun

Answer 10

It contains mitochondria

Question 11

What does the sediment contain the third time it is spun

Answer 11

It contains ribosomes,membranes,etc.