Cell migration Flashcards
(12 cards)
What are some examples of cell migrating ?
1) Wiring of the human brain
2) Epithelial cells migrating to heal wound and extravasation of white blood cells
3) slow migration of endothelial cells that line blood vessels
4) Inappropriate migration of cancer cells
What are filopodia made out of?
Actin filaments formed by formins
What is leading edge or lamellipodium made out of?
branched actin filaments by Arp 2/3 complex
What are stress fibres?
Contractile microfilaments that attach to the substratum of the cell through specialised regions called focal adhesion or focal contacts
What are the four phases of cell movement ?
1) Extension
2) Adhesion
3) Translocation
4) De-adhesion and endocytic recycling
What actions are required for leading edge of a motile cell?
Action of ARP 2/3 complex, forming and bundling proteins that all push the membrane forward
What do cells adhere to when moving?
Focal adhesions
What proteins are involved in focal adhesion ?
Integrins
What do integrins connect ?
Extracellular matrix and actin filaments
What happens to focal adhesions once they reach the back of the cell?
They get internalised by endocytosis and are brought to the front to make new adhesions
What produces the motile force of stress fibres?
Actin binding motor proteins called myosins
What happens during translocation?
The contents of the cell (organelles nucleus) are squeezed forward by cortical contraction of the cytoskeleton
