Cell Pathology

Question 1

Haematoxylin & Eosin
A) Nuclei,
B) Cytoplasm & collagen
C) Membranes, muscle, keratin
D) Erythrocytes

Answer 1

Blue
Pale Pink
Bright pink
Orange red

Question 2

What does Masson Trichrome stain

Answer 2

Connective tissue esp. collagen

Question 3

Masson Trichrome staining
A) Nuclei
B) Muscle, epithelia
C) collagen
D) red blood cells

Answer 3

Blue (Hx)
Pink (Ponceau red)
Green (light green)
Scarlet (Acid Fuchsin)

Question 4

What does Verhoeffs Van Gieson stain?

Answer 4

Elastin fibers
E.g in arteries, lungs, skin

Question 5

Verhoeffs Van Gieson
A) Elastin fibres, nuclei
B) Cytoplasm, muscle
C) Collagan

Answer 5

Black
Yellow
Red

Question 6

What does Gordan & Sweets Silver stain?

Answer 6

Reticular fibres (Collagen type III) - in liver, spleen, lymph nodes.
Many in basement membrane.

Question 7

Gordan & Sweets Silver stain
A) Reticulin
B) Background

Answer 7

Black
Green.

Question 8

Is G&S Stain argyrophillic or argentaffin stain?

Answer 8

Argyrophillic

Question 9

What does Perls Prussian Blue stain?

Answer 9

Iron deposits (hemosiderin) - commonly used in liver, bone marrow and spleen samples.

Question 10

Perls Prussian Blue
A) Iron deposits (hemosiderin)
B) Background

Answer 10

Blue
Pink/Red

Question 11

What does Alcian Blue/Periodic Acid Schiff stain?

Answer 11

Glycogen, neutral and acid mucins.

Question 12

Alcian Blue/Periodic Acid Schiff
A) Acidic mucins (glycoaminoglycans)
B) Neutral mucins/glycogen/basement membrane/fungi
C) Nuclei

Answer 12

Sky Blue
Magenta (PAS)
Deep Blue

Question 13

What does Congo Red stain?

Answer 13

Amyloid deposits

Question 14

Congo red
A) Amyloid deposits
B) Nuclei

Answer 14

Pink-red
Blue

Question 15

What does Grocott’s Methenamine Silver stain?

Answer 15

Fungi - Pneumocystis, Candida

Question 16

Grocott’s Methenamine Silver
A) Fungal cell walls
B) Background

Answer 16

Black
Green