cells Flashcards
(55 cards)
What organelles are present in prokaryotic cells but not eukaryotic cells?
flagellum, DNA loop, centrioles, plasmids
What is the function of the cell surface membrane?
controls what enters and leaves the cell.
What is the function of the nucleus?
controls protein synthesis by mRNA and tRNA. Manufactures RNA and ribosomes.
What is the function of the mitochondrion?
Site of aerobic respiration - production of ATP cells with high levels of metabolic activity.
What is the function of chloroplast?
Site of photosynthesis.
What is the function of golgi apparatus?
Add carbohydrates to proteins, produce secretary enzymes, secrete carbohydrates, transport modify and store lipids, form lysosomes.
What is the function of lysosomes ?
Isolation of enzymes before release to outside of the cell, hydrolyse material ingested by phagocytic cells, release enzymes to destroy material around cell.
What is the function of ribosomes?
protein synthesis
What is the function of rough endoplasmic reticulum?
protein synthesis
What is the function of smooth endoplasmic reticulum?
synthesis and store of lipids and carbohydrates.
What is the function of the cell wall?
mechanical strength to prevent bursting from osmotic pressure, allow water to pass along it.
What is the function of the cell vacuole?
support herbaceous parts of woody plants by making cells turgid, contains sugars and amino acids that may act as a temporary food store.
What is differentiation?
The process by which cells become specialised for different functions.
Define tissue.
A group of similar cells which carry out a specific function.
What are the advantages of a light microscope?
real colours, can use a living sample
What are the disadvantages of a light microscope?
lower resolution, can not see smaller organelles, lower magnification.
what are the advantages of scanning electron microscope?
3D image, thick specimens, false colour
What are the disadvantages of the scanning electron impact microscope?
Sample must be in a vacuum, cant view living samples, lower resolution than T.E.M
What are the advantages of a transmission electron microscope?
higher resolution so can see ultrastructure
What are the disadvantages of the transmission electron microscope?
very thin specimen, black & white image, need a vacuum, image contains artefacts, 2D image.
What is the equation for magnification?
image size = actual size x magnification
Explain how you would prepare a stained temporary mount.
1) use a knife to cut a thin slice of the specimen
2) use forceps to place the specimen on the slide.
3) add iodine
4) use a mounted needle to lower the cover slip onto the slide.
What is cell fractionation used for?
Separating different organelles of a cell so they can be studied in detail.
What is the first stage of cell fractionation?
Tissue is cut up and kept in a cold, isotonic buffered solution.