Cells/Centrifugation Flashcards
Parts of a prokaryotic cell?
Cell wall, capsule, cell membrane, flagellum, circular DNA, plasmid, ribosomes, cytoplasm, circular DNA.
Nucleus
(incl. nuclear envelope, nuclear pores, nucleoplasm, chromatin, nucleolus) Storage site of genetic information, the site of DNA replication and its transcription to RNA (mRNA and tRNA). Manufacture ribosomal RNA and ribosomes.
Plasmid
Small circles of DNA, used to exchange DNA between bacterial cells.
Circular DNA
Contains genetic information to let cells replicate. Not associated with proteins.
Mitochondria
Mitochondria release energy/ produce ATP
- From aerobic respiration
- Double membrane – controls entry and exit of material
- Contain cristae (shelf like extensions of inner membrane – large SA for enzymes in respiration to attach to) & matrix (contains enzymes for respiration)
Cell wall
Made of peptidoglycan (Murein). Protects cell against damage.
Cytoplasm
Contains enzymes for metabolic reactions.
Capsule
Thick polysaccharide layer outside of the cell for protection/helps groups of bacteria stick together for more protection.
Ribosomes
Ribsosomes – 80S type in eukaryotic cells (bigger)/70S in prokaryotic.
-Make proteins (remember enzymes are proteins)
Flagellum
Tail for movement. Helps spin cell through fluids.
Rough endoplasmic reticulum
Surface ribosomes bind to for protein synthesis. Provides pathway for transport of materials around the cell.
Smooth endoplasmic reticulum
Makes, stores and transports lipids and carbohydrates.
Comparing prokaryotic and eukaryotic cells- like for like
-Bacteria don’t have a nucleus/nuclear membrane, eukaryote does;
-Bacteria don’t have membrane-bound organelles, eukaryote does;
-Bacteria don’t have mitochondria, eukaryote does;
-Bacteria has 70S/smaller ribosomes, eukaryote has 80S/larger;
Bacteria cell- have murein/peptidoglycan cell wall, eukaryotes do not-.
Bacterial cells - smaller than eukaryotic cells;
Bacteria - circular DNA, eukaryotes - linear DNA;
Bacteria may have capsules, eukaryotes don’t;
Golgi body
Modifies polypeptide chains into mature proteins. Sorts and ships lipids for secretion or use within the cell. Makes secretory enzymes. Forms glycoproteins. Modified proteins and lipids are transported in golgi vesicles.
Centrifugation
Helps to separate organelles. Most dense organelles separate 1st, forming a pellet, which is removed. Supernatant contains other organelles –can spin again.