Cells, Viruses and Reproduction of Living Things Flashcards
(129 cards)
Cell theory
- Cells are the basic building blocks of life.
- They are the fundamental units of structure, function and organisation in living organisms.
-Cell theory is a unifying concept which links all life forms on earth together.
Structure
Function
Organisation
TISSUES
Group of similar cells
Working together
To perform a particular function
ORGANS
Groups of different tissues
Structured so they work together
To perform a particular function
SYSTEMS
Group of different organs
Working together to carry out large scale functions
Examples include the digestive system and the nervous system
Prokaryotic cells
Prokaryotic simply means before the nucleus. Prokaryotic organisms are always single celled.
Basic properties of prokaryotic cells
Single celled organisms
DNA suspended freely in the cytoplasm
It does not have a nucleus
Do not have membrane bound organelles
Smaller than eukaryotic cells
Organelles within prokaryotic cells:
NUCLEOID
Genetic material consists of a single strand of DNA which is often circular
The DNA is folded and coiled and the area becomes very dense with DNA
This area is known as the nucleoid
Organelles within prokaryotic cells:
PLASMIDS
Smaller circles of DNA
Code for a particular aspect of the cell such as a toxin
Is able to reproduce independently of the nucleoid
Can be transferred from one bacterium to another in a form of sexual reproduction using the pilli
Organelles within prokaryotic cells:
70S RIBOSOMES
Not membrane bound
Involved in protein synthesis
Made up of two smaller sub-units
Larger 50s unit and a smaller 30s unit
Smaller than the 80s ribosomes found in eukaryotic cells
Organelles within prokaryotic cells:
CELL WALLS
All bacterial cells have a cell wall
Contents of the cell are usually hypertonic so water moves into the cell by osmosis
The wall prevents swelling and bursting, maintain the shape of the bacterium and it gives support and protection to the contents of the cell
All have some sort of layer of peptidoglycan
Organelles within prokaryotic cells:
SLIME CAPSULE
Surrounds the cell walls and covers cell markers, making the cell hard to identify
Therefore it protects the bacterium from phagocytosis
This feature is present in all prokaryotic cells and enables the cell to more easily become pathogenic
Bacterial cells
Bacterial cells can either be Gram positive or Gram negative.
Which one they are depends on the type of cell wall it has
GRAM POSITIVE BACTERIA
Have a thick layer of peptidoglycan
Contain chemicals such as teichoic acid
Thick layer resists staining
Therefore leaving a purple/blue colour after staining
GRAM NEGATIVE BACTERIA
Thin layer of peptidoglycan
No teichoic acid
Thin layer of peptidoglycan is easily broken down
Therefore leaving the cell red in colour after staining
How are antibiotics be used to kill bacteria?
Antibiotics can be used to treat most pathogenic bacteria.
They kill these bacterial cells by targeting features specific to bacterial cells, preventing them from reproducing.
Which antibiotic is used depends upon which type of bacteria is present.
If the wrong antibiotic is prescribed the person will see no benefit.
Procedure of gram staining
Firstly, crystal violet is used to stain over a heat fixed culture
After a minute,the stain is poured off and the slide is rinsed with water
Then, iodine solution is added and removed after a minute
Alcohol is then added. Liposaccharides are soluble in alcohol, whereas peptidoglycan is not, so gram negative bacteria is decolourised.
The final step of the prosecure is counterstaining with red safranin for another minute. The sample is then dried and examined
What colour does gram positive bacteria appear?
Under the microscope, gram positive bacteria appears violet/purple
What colour does gram negative bacteria appear?
Under the microscope, gram negative bacteria appears red
What do antibiotics usually target?
Antibiotics usually target features of bacterial cells that differ from eukaryotic cells, including the bacterial cell walls and 70s ribosomes
Explain why some antibiotics are effective against gram positive bacteria and not gram negative bacteria?
(Beta-lactam antibiotics)
Some antibiotics, such as beta-lactam antibiotics, inhibit the formation of the peptidoglycan layer of the cell wall.
As a result, they are effective against gram positive bacteria as they have a thick peptidoglycan layer on the surface of the cell.
However, they are less effective on gram negative bacteria, as their peptidoglycan layer is hidden and is less vital to the wall structure
Explain why some antibiotics are effective against gram positive bacteria and not gram negative bacteria?
(Glycopeptide antibiotics)
Glycopeptide antibiotics are large polar molecules that cannot penetrate the outer membrane layer of gram negative bacteria.
However, they are very effective against gram positive bacteria, even the ones that have developed resistance to many other antibiotics
Explain why some antibiotics are effective against gram negative bacteria and not gram positive bacteria?
Polypeptide antibiotics are rarely used because they can have serious side effects.
These are very effective against gram negative bacteria because they interact with the phospholipids of the outer membrane
They do not affect gram positive bacteria
Explain why some antibiotics are effective against both gram negative bacteria and gram positive bacteria?
Most other antibiotic affect both gram positive and gram negative bacteria because they target common processes such as protein synthesis by the ribosomes.
They only target prokaryotic ribosomes, not eukaryotic ribosomes.
Cocci
spherical bacteria