cellular respiration Flashcards

(55 cards)

1
Q

What does the First Law of Thermodynamics state?

A

Energy cannot be created or destroyed, only transformed.

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2
Q

How does the First Law of Thermodynamics apply to living organisms?

A

Living organisms must obtain and convert external energy (from food or sunlight) to fuel biological processes.

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3
Q

What is the Second Law of Thermodynamics?

A

Energy transformations increase the overall entropy of the universe.

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4
Q

How do living systems maintain low entropy despite the Second Law of Thermodynamics?

A

By releasing heat and waste, increasing entropy in their environment while maintaining internal order.

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5
Q

Define an exergonic reaction.

A

A reaction that releases energy, such as cellular respiration.

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6
Q

What is a catabolic process? Give an example.

A

A catabolic process breaks down molecules to release energy, like breaking down glucose in cellular respiration.

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7
Q

What is an endergonic reaction?

A

A reaction that requires an input of energy to proceed, such as photosynthesis.

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8
Q

What is an anabolic process? Give an example.

A

An anabolic process builds complex molecules from simpler ones, like protein synthesis.

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9
Q

What does it mean for reactions to be ‘coupled’ in biological systems?

A

An exergonic reaction provides energy to drive an endergonic reaction.

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10
Q

Give an example of coupled reactions in cells.

A

ATP hydrolysis (exergonic) couples with muscle contraction or active transport (endergonic).

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11
Q

What role does ATP play in cellular work?

A

ATP releases energy through hydrolysis to power cellular activities like muscle contraction and active transport.

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12
Q

How does ATP release energy for cellular processes?

A

By breaking its high-energy phosphate bonds in an exergonic reaction.

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13
Q

What is Gibbs Free Energy (AG)?

A

AG measures the spontaneity of a reaction; negative AG indicates a spontaneous reaction.

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14
Q

How do we calculate AG in a reaction?

A

G = ДН - TAS, where AH is enthalpy change, T is temperature in Kelvin, and AS is entropy change.

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15
Q

If AG is positive, what does it mean for a reaction?

A

The reaction is non-spontaneous (endergonic) and requires energy input.

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16
Q

What does a negative AG indicate?

A

The reaction is spontaneous (exergonic) and releases energy.

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17
Q

What type of macromolecule are enzymes?

A

Enzymes are proteins.

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18
Q

What determines the shape of an enzyme?

A

The enzyme’s shape is determined by its amino acid sequence and how it folds into its tertiary structure.

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19
Q

What is the function of the enzyme’s active site?

A

The active site binds to substrates, facilitating the chemical reaction.

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20
Q

Describe the ‘lock and key’ model of enzyme function.

A

This model suggests that the enzyme’s active site perfectly fits the substrate, like a key fitting a lock.

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21
Q

What is the ‘induced fit’ model of enzyme function, and why is it more accurate?

A

This model proposes that the enzyme’s active site changes shape to bind more closely with the substrate, making it more accurate for how enzymes work.

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22
Q

What are enzyme ‘co-factors’? Give an example.

A

Co-factors are non-protein molecules that assist enzymes, such as zinc or magnesium ions.

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23
Q

How do enzymes accomplish biological catalysis?

A

Enzymes lower the activation energy of reactions, speeding them up without being consumed.

24
Q

What is the difference between catalyzed and uncatalyzed reactions?

A

Catalyzed reactions proceed faster because enzymes lower the activation energy.

25
Define activation energy.
Activation energy is the energy required to start a reaction.
26
What is enzyme saturation?
Enzyme saturation occurs when all active sites are occupied, so increasing substrate concentration won't speed up the reaction further.
27
How can cells influence enzyme activity?
Cells can regulate enzyme activity through pH, temperature, inhibitors, and activators.
28
How can enzymes be activated?
Enzymes can be activated by binding to cofactors or by modifications like phosphorylation.
29
How can enzymes become inactivated?
Enzymes may become inactivated through changes in pH, temperature, or by binding to inhibitors.
30
Define allosteric regulation.
Allosteric regulation involves a molecule binding to an enzyme at a site other than the active site, altering its activity.
31
What is the significance of allosteric regulation in enzyme activity?
Allosteric regulation allows cells to fine-tune enzyme activity, often in response to metabolic needs.
32
How do competitive inhibitors affect enzyme activity?
Competitive inhibitors bind to the active site, blocking the substrate from binding.
33
How do non-competitive inhibitors affect enzyme activity?
Non-competitive inhibitors bind to an enzyme away from the active site, changing its shape and reducing function.
34
How can environmental conditions affect enzyme function?
Conditions like temperature and pH can change enzyme structure, affecting their activity.
35
What happens when an enzyme is 'denatured'?
The enzyme loses its structure and function due to disruption of bonds, affecting secondary, tertiary, or quaternary structure.
36
What does it mean for an enzyme to be 'renatured'?
Renaturation occurs when an enzyme regains its shape and activity after being returned to favorable conditions.
37
How does pH impact enzyme activity?
pH changes can disrupt ionic bonds, altering the enzyme's shape and efficiency.
38
Why does increased temperature speed up enzyme-catalyzed reactions?
Higher temperatures increase molecular motion, boosting reaction rates until the enzyme denatures at too high a temperature.
39
What happens to enzymes at excessively high temperatures?
Enzymes denature, losing their structure and function.
40
How could you experimentally measure enzyme function?
By tracking changes in substrate or product concentration over time, often using a spectrophotometer.
41
Describe the structure of the mitochondrion.
The mitochondrion has an outer membrane, inner membrane with folds (cristae), and a matrix where cellular respiration occurs.
42
Where do glycolysis, Krebs cycle, and ETC take place within the cell?
Glycolysis occurs in the cytoplasm, the Krebs cycle in the mitochondrial matrix, and the ETC along the inner mitochondrial membrane.
43
Why does energy metabolism occur in small steps?
Small steps prevent the release of too much energy at once, allowing efficient energy capture as ATP.
44
What are the four pathways in cellular respiration?
Glycolysis, pyruvate oxidation, Krebs cycle, and the electron transport chain (ETC).
45
What are the inputs and outputs of glycolysis?
Inputs: glucose, ATP, NAD+; Outputs: pyruvate, ATP, NADH.
46
What are the inputs and outputs of pyruvate oxidation?
Inputs: pyruvate, NAD+; Outputs: acetyl-CoA, NADH, CO2.
47
What are the inputs and outputs of the Krebs cycle?
Inputs: acetyl-CoA, NAD+, FAD; Outputs: CO2, ATP, NADH, FADH2.
48
What are the inputs and outputs of the electron transport chain (ETC)?
Inputs: NADH, FADH2, O2; Outputs: ATP, H2O.
49
Describe oxidative phosphorylation.
It includes the ETC and chemiosmosis, where ATP is generated as protons flow through ATP synthase.
50
What are the two types of fermentation?
Lactic acid fermentation and alcoholic fermentation.
51
How does fermentation compare to oxidative phosphorylation?
Fermentation is less efficient, producing only 2 ATP per glucose compared to ~30-32 in oxidative phosphorylation.
52
How many ATP are produced from one glucose in oxidative phosphorylation?
About 30-32 ATP.
53
How many ATP are produced from fermentation?
Only 2 ATP per glucose.
54
How does the breakdown of fats, proteins, and carbs produce ATP?
These macromolecules are converted into intermediates that enter glycolysis, the Krebs cycle, or ETC for ATP production.
55
How do bacteria generate ATP without mitochondria?
Bacteria use their cell membrane for processes like the electron transport chain to produce ATP.