Ch. 15 Translation Flashcards

(38 cards)

1
Q

Translation

A

RNA –> proteins

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2
Q

amino acids

A

building blocks for proteins

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3
Q

what are the components that every amino acid has in common?

A
  1. amino group
  2. carboxyl group
  3. hydrogen
  4. R group
  5. carbon (center)
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4
Q

what are the different types of R groups?

A
  1. nonpolar
  2. polar
  3. aromatic (ring structure)
  4. positively charged
  5. negatively charged
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5
Q

how many different amino acids are there?

A

20

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6
Q

what type of bonds join amino acids?

A

peptide bonds

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7
Q

define “polypeptide”

A

a string of amino acids (a protein)

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8
Q

will mutation be greater when swapping one type of R group for a DIFFERENT type of R group? or will it be greater when swapping same R group for same type of R group?

A

swapping different TYPES of R groups will cause greater mutations in secondary protein structure

  • swapping same TYPE of R group will not cause as great of a mutation
    (ex. swapping polar for non-polar)
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9
Q

where do peptide bonds form between amino acids?

A

dehydration reactions from N terminus –> C terminus

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10
Q

primary structure of amino acids

A

polypeptide chain

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11
Q

secondary structure of amino acids

A

areas of folding or coiling within a protein; examples include alpha helices and pleated sheets, which are stabilized by hydrogen bonding

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12
Q

tertiary structure of amino acids

A

the final three-dimensional structure of a protein, which results from a large number of non-covalent interactions between amino acids.

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13
Q

quaternary structure of amino acids

A

several different polypeptide strands together, all folding together into complex structure, gain of function

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14
Q

genetic code consists of _____.

A

triplets of bases
(one triplet = one amino acid)
each triplet is a CODON

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15
Q

define “degeneracy” of amino acid codes

A

more than one triplet encodes for the same aa

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16
Q

synonymous codons

A

codons that specify for the same amino acid

17
Q

where are codons found?

18
Q

where are anticodons found?

A

tRNA

  • complementary to codons
  • responsible for “reading” the mRNA
19
Q

what is the wobble position?

A

the third base pair of a triplet is usually not detrimental because it will still code for the same protein as long as the first 2 base pairs are the same
-allows for degeneration of the code

20
Q

the genetic code is ______.

A

non overlapping

each triplet of 3 is read separately

21
Q

for any stretch of DNA, there are ____ possible reading frames.

A

3

this depends on where you begin to read the DNA

22
Q

how do we know which reading frame to start reading on?

A

start codons!

23
Q

what is the start codon?

24
Q

what are the stop codons? (or non-sense codons)

A

UAA
UAG
UGA
stop codons have NO AMINO ACIDS and no tRNAs associated with them

25
what is an "open reading frame"? (ORF)
goes from the start codon to the stop codon
26
which 3 types of RNA are involved with translation?
mRNA tRNA ribosomes (rRNA and proteins)
27
what is the structure of tRNA?
1. cloverleaf secondary structure 2. presence of rare bases 3. amino acid attachment site (CCA) 4. anticodon arm (pairs with corresponding mRNA sequence, reads the mRNA by complementary base pairing)
28
what is tRNA charging?
the process of attaching an amino acid to the appropriate tRNA - so that tRNA can "read" mRNA in a complementary and antiparallel fashion * requires ATP*
29
what enzyme carries out tRNA charging?
aminoacyl-tRNA synthetases
30
mature ribosomes are made of many proteins associated with ____.
4 RNA subunits
31
ribosomes are _______.
translators
32
_____% of the total cellular RNA is ribosomes
80%
33
ribosomes consist of a ___ subunit and a ____ subunit
small | large
34
ribosomes have ___ distinct sites
3
35
what are the 3 main sites on a ribosome?
1. E exit site (tRNA dissociates) 2. P peptidyl site (peptide bonds forms) 3. A aminoacyl site (charged tRNA attaches)
36
what is a release factor?
comes in to stop elongation once a stop codon is reached | separate strands
37
multiple ribosomes translate the same mRNA in a structure called a ____.
polyribosome
38
how can we tell that translation is occurring in a bacteria or an animal?
translation happens in cytoplasm in animals | transcription and translation happen together in bacteria (also no RNA processing)