Ch4.Culture Media

Question 1

What is Culture media?

Answer 1

It is a medium containing nutrients, growth factors etc in which microbes can grown in.

Question 2

What are the components of Culture media?

Answer 2

• Nutrients and energy sources
  (H2O, CO2,NO2 sources)
• Essential minerals
  (Na,K,Cl etc.)
• Selective agent
  (Antimicrobial agents)
• Gelling agent
  (Agar,gelatine)

Question 3

What are the types of culture media based on CONSISTENCY?

Answer 3

a) Liquid/ nutrient broth- (no agar)
b) Semi solid media- (Broth+half qty agar)
c)Solid media- (Agar)

Question 4

Which media is used for fungi and which one isn’t?

Answer 4

-Liquid media/ nutrient broth used,
Solid media not used

-secondary metabolites can be obtained frm liquid media.

Question 5

What are the types of media based on CHEMICAL COMPOSITION?

Answer 5

1) Natural/empirical
2)Semi-synthetic
3) Synthetic

Question 6

Natural/empirical media’s chemical composition is __________.

Answer 6

NOT KNOWN.

Question 7

Semi-synthetic media’s chemical composition is __________.

Answer 7

PARTIALLY KNOWN.

Question 8

Synthetic media’s chemical composition is __________.

Answer 8

FULLY KNOWN.

Question 9

2 egs. Of natural media

Answer 9

Milk, blood

Question 9

2 egs. Of natural media

Question 10

2 egs. Of semi synthetic media

Answer 10

Potato dextrose agar, Cornmeal agar

Question 11

2 egs. Of synthetic media

Answer 11

Richards soln. , Glucose medium

Question 12

Types of routine lab media

Answer 12

Basal, Enriched, Selective, Indicator, Transport and Storage.(BESTIS)

Question 13

Uses of basal media and few egs.

Answer 13

Used for bacteria growth - limited nutrients provided.
Egs. Nutrient broth/agar, peptone water.

Question 14

Description of enriched media and few egs.

Answer 14

Enriched by addition of blood,serum,egg.
Egs. Lowenstein Jensen media, Blood agar media.

Question 15

Uses of selective media and few egs.

Answer 15

•Favour growth of particular bacteria by inhibiting growth of undesirable bacteria or fungi.
•Antibiotic added.
Egs.

Question 16

Why is an indicator media used? Few egs

Answer 16

Media used as indicator is added nd growth of particular organism causes colour change.
Eg. Mc Leod’s media, Wilson-Blair media

Question 17

Why is a transport media used? Few egs.

Answer 17

Used when specimen can’t be cultured soon after collection and used to transport the sample.
Eg. Cary-Blair media, Amieo media, Stuart media

Question 18

Why is an storage media used? Few egs

Answer 18

Used to store microbes for a long period of time.
Eg. Egg saline media, Robertson’s cooked meat broth