Ch7: Plating Media.

Question 1

1. Bile esculin agar (BEA).

Answer 1

Differential isolation and
presumptive identification of
group D streptococci and enterococci.

Question 2

2. Bile esculin azide agar with vancomycin.

Answer 2

Selective and differential for cultivation of vancomycin-resistant enterococci from clinical and surveillance specimens.

Question 3

3. Blood agar.

Answer 3

Cultivation of
nonfastidious microorganisms,
Determination of hemolytic reactions.

Question 4

4. Bordet-Gengou agar.

Answer 4

Isolation of Bordetella pertussis and Bordetella parapertussis.

Question 5

5. Brain heart infusion

agar or broth.

Answer 5

Cultivation of

fastidious organisms.

Question 6

6. Buffered charcoal-yeast 
extract agar (BCYE).

Answer 6

Enrichment for Legionella spp.
Supports the growth of
Francisella and Nocardia spp.

Question 6

7. Buffered charcoal-yeast extract (BYCE) agar with antibiotics.

Answer 6

Enrichment and selection for Legionella spp.

Question 7

8. Burkholderia cepacia selective agar.

Answer 7

For recovery of B. Cepacia from cystic fibrosis patients.

Question 8

9. Campy-blood agar.

Answer 8

Selective for

Campylobacter spp.

Question 9

10. Campylobacter thioglycollate broth.

Answer 9

Selective holding medium for recovery of Campylobacter spp.

Incubated at 4oC for cold-enrichment.

Question 10

11. CDC anaerobe

5% sheep blood agar.

Answer 10

Improved growth of obligate, slow-growing anaerobes.

Question 11

12. Cefoperazone, vancomycin, amphotericin (CVA) medium.

Answer 11

Selective medium for isolation of Campylobacter spp.

Question 12

13. Cefsulodin-irgasan-novobiocin (CIN) agar.

Answer 12

Selective for Yersinia spp.;

may be useful for isolation of Aeromonas spp.

Question 13

14. Chocolate agar.

Answer 13

Cultivation of fastidioius microorganisms such as Haemophilus spp., Brucella spp. and pathogenic Neisseria spp.

Question 14

15. Chromogenic media.

Answer 14

Designed to optimize growth and differentiate a specific type of organism. Chromagars are routinely used in the identification of yeasts, methicillin-resistant Staphylococcus aureus (MRSA), and a variety of other organisms.

Question 15

16. Columbia colistin-nalidixic acd (CNA) AGAR.

Answer 15

Selective isolation of

gram-positive cocci.

Question 16

17. Cystine-tellurite blood agar.

Answer 16

Isolation of C. diphtheriae.

Question 17

18. Eosin methylene blue (EMB) agar (Levine).

Answer 17

Isolation and differentiation of lactose-fermenting and non-lactose-fermenting enteric bacilli.

Question 18

19. Gram-negative broth (GN)

Answer 18

Selective (enrichment) liquid medium for enteric pathogens.

Question 19

20. Hektoen enteric (HE) agar.

Answer 19

Differential, selective medium for the isolatino and differentiation of Salmonella and Shigella spp. from other gram-negarive enteric bacilli.

Question 20

21. Loeffler’s medium

Answer 20

Isolation and growth of Corynebacterium.

Question 21

22. MacConkey agar.

Answer 21

Isolation and differentiation of lactose fermenting and non-lactose-fermenting enteric bacilli.

Question 22

23. MacConckey sorbitol agar.

Answer 22

For the selection and differentiation of E. coli 0157:H7 in stool specimens.

Question 23

24. Mannitol salt agar.

Answer 23

Selective differentiation of staphylococci.

Question 24

25. Nyw York City (NYC) agar.

Answer 24

Selective for Neisseria gonorrhoeae; | supports the growth of Ureaplasma urealyticum and some Mycoplasma spp.

Question 25

26. Phenylethyl alcohol (PEA) agar?

Answer 25

Selective isolation of aerobic gram-positive cocci and bacilli and anaerobic gram-positive cocci and negative bacilli.

Question 26

27. Regan Lowe?

Answer 26

Enrichment and selective medium for isolation of Bordetella pertussis.

Question 27

28. Salmonella-Shigella (SS) agar?

Answer 27

Selective for Salmonella and some Shigella spp.

Question 28

29. Schaedler agar?

Answer 28

Nonselective medium for recovery of anaerobes and aerobes. | Selective for Campylobacter and Helicobacter spp.

Question 29

30. Selenite broth?

Answer 29

Enrichment of isolation of Salmonella spp.

Question 30

31. Skirrow agar?

Answer 30

Selective for Campylobacter spp.

Question 31

32. Streptococcal selective agar (SSA)?

Answer 31

Selective for Streptococcus pyogenes and Streptococcus agalactiae.

Question 32

33. Tetrathionate broth?

Answer 32

Selective for Salmonella and Shigella spp. except S. typhi.

Question 33

34. Thayer-Martin agar (modified Thayer Martin)?

Answer 33

Selective for N. gonorrhoeae and N. meningitidis. | Supports growth of Francisella and Brucella spp.

Question 34

35. Thioglycollate broth?

Answer 34

Supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms,

Question 35

36. Thiosulfate citrate-bile salts (TCBS) agar?

Answer 35

Selective and differential for Vibrio spp.

Question 36

37. Todd-Hewitt broth supplemented with antibiotics (LIM)?

Answer 36

Selection and enrichment for Streptococcus agalactiae in female genital specimens.

Question 37

38. Trypticase soy broth (TSB)?

Answer 37

Enrichment broth used for subculturing various bacteria from primary agar plates?

Question 38

39. Xylose lysine desoxycholate (XLD) agar?

Answer 38

Isolation and differentiation of Salmonella and Shigella spp. from other gram-negative enteric bacilli.