Chapter 1,2,3,4

Question 1

Which Factor may cause a blood smear to be too thin?

Answer 1

the angle of the spreader is low

Question 2

What is a supravital stain?

Answer 2

A method of staining used to examine living cells that have been removed from an organism. IE. New methylene blue

Question 3

What is an example of a supravital stain?

Answer 3

New methylene blue

Question 4

What is the Normal PH of blood?

Answer 4

Between 7.35 and 7.45

Question 5

The stain most used for a DIFFERENTIAL SMEAR?

Answer 5

Wright’s stain

Question 6

When performing a white blood count, what does the DILUENT do?

Answer 6

1. Hemolyzes the red blood cells
2. distributes the cells evenly
3. stains the nucleus of the white blood cells

**note: first 2 are most important

Question 7

The stain most used for Reticulocytes is?

Answer 7

New Methylene blue

Question 8

Where are the blood antibodies located? Where are the blood Antigens located?

Answer 8

Antibodies are located in the plasma, antigens are located on the surface of the RBC.

Question 9

The movement of leukocytes through the pores of the capillaries is:

Answer 9

diapedesis movement

Question 10

In vivo means

Answer 10

in the living body

**remember vivir in spanish means living hence the word vivo

Question 11

When setting up ESR, it is important to maintain certain standards/environment:

Answer 11

1. ESR tube must be vertical
2. Kept away from centrifuge
3. Not kept in an area that is too hot or moist

*NOTE: #1 important

Question 12

How long is a microhematocrit centrifuged for and at what RPM?

Answer 12

5 minutes at 10000RPM

Question 13

What units is ESR recorded?

Answer 13

mm/hr

Question 14

Any Blood samples must be gently inverted or mixed thoroughly to

Answer 14

ensure even distribution of all components

Question 15

The test that counts immature RBCs?

Answer 15

Reticulocyte count

Question 16

What reagent is used to dilute blood for Hemoglobin estimation?

Answer 16

Drabkin’s reagent

Remember: drabkin’s reagent is dangerous because it contains CYANIDE.

Question 17

What is the black tube(Westergren) used for?

Answer 17

ESR

Question 18

What do we cleanse the area with? before performing a venipuncture or capillary puncture?

Answer 18

We use the alcohol swabs which are 70% ALCOHOL

Question 19

When counting WBCs for a differential count, how many cells do we count?

Answer 19

100

Question 20

At what angle do we seal a capillary tube with clay for a microhematocrit?

Answer 20

90 degrees

Question 21

The immunohematology test that is incubated after collection is

Answer 21

cold agglutinins

Question 22

After a blood film(smear) is made

Answer 22

we allow it to airdry slowly

Question 23

what angle do we hold the spreader slide when making a blood smear?

Answer 23

25 to 30 degrees

Question 24

poikilocytosis

Answer 24

abnormally shaped RBCs

Question 25

anisocytosis

Answer 25

abnormally sized RBCs

Question 26

crenated cell

Answer 26

has scalloped edges

Question 27

normocyte

Answer 27

normal sized RBC

Question 28

microcyte

Answer 28

abnormally small RBC

Question 29

macrocyte

Answer 29

abnormally large RBC

Question 30

antibody

Answer 30

fights antigen

Question 31

phagocytosis

Answer 31

eats waste such as deteriorated cells or old cells or foreign material

Question 32

What is the difference between serum and plasma

Answer 32

They both contain the same analytes but plasma has clotting factors such as fibrinogen and serum doesn't

Question 33

Where do you read the results of a microhematocrit?

Answer 33

Top of the red cell column, do not include buffy coat layer

Question 34

Where are RBCs, WBCs, reticulocytes made?

Answer 34

RED BONE MARROW

Question 35

What are the immature white blood cells called?

Answer 35

Band cells

Question 36

What are the immature red blood cells called ?

Answer 36

reticulocytes

Question 37

Which anemia where the red bone marrow fails to produce RBCs?

Answer 37

aplastic anemia

Question 38

Which anemia where the RBCs appear microcytic, hypochromic and some become target cells?

Answer 38

thalassemia *remember: rbcs becoming target cells-think thalassemia

Question 39

Which anemia is caused by abnormal "HGB S"?

Answer 39

Sickle Cell

Question 40

The most common type of anemia is

Answer 40

Iron deficiency anemia

Question 41

Before reading the hematocrit, we must

Answer 41

centrifuge

Question 42

The destruction of red blood cells or red blood cel wall is called

Answer 42

hemolysis

Question 43

Thrombocytes are ___________ therefore thrombocytopenia is ______________________.

Answer 43

platelets, decrease in platelets

Question 44

Erythroblastosis fetalis

Answer 44

a hemolytic anemia in the fetus caused when the mother's blood type is Rh- and she is pregnant with a Rh+ baby.