Chapter 1: Aba Ginoong Maria (Remix)

Question 1

Fixative recommended for center nervous tissue and general post-mortem tissues

Answer 1

10% Formal Saline

Question 2

Excellent microanatomic fixative for pituitary gland

Answer 2

Zenker-Formol (Helly’s Solution)

Question 3

Recommended mainly for tumor biopsies especially for the skin

Answer 3

Heidenhain’s Susa Solution

Question 4

Recommended for demonstration of chromatin, mitochondria, mitotic figure, RBC’s and golgi bodies

Answer 4

Regard’s (Muller’s) Fluid

Question 5

It demonstrates rickettsiea and other bacteria

Answer 5

Orth’s fluid

Question 6

Recommended for acid mucopolysaccharides

Answer 6

Lead Fixatives

Question 7

Fixes connective tissue mucin

Answer 7

Lead fixatives

Question 8

Recommended fixation of embryos and pituitary biospies

Answer 8

Bouin’s Solution

Question 9

Excellent fixative for glycogen

Answer 9

PICRIC ACID FIXATIVES

Brasil’s Alcoholic Picroformal Fixative

Question 10

Considered to be the most rapid fixative

Answer 10

Carnoy’s fixative

Question 11

Excellent fixative for nuclear structures (e.g chromosomes)

Answer 11

Flemming’s Solution

Question 12

The fixative used at ice cold temperature ranging from -5 degrees celsius to 4 degree celsius

Answer 12

Acetone

Question 13

The fixative contains mercuric chloride and glacial acetic acid

Answer 13

Zenker’s fluid

Question 14

Commonly used for bone marrow biopsies

Answer 14

B-5 fixative

Question 15

Cheap, readily available, easy to prepare, and widely used fixative

Answer 15

10% formal saline/formalin
Buffered formalin

Question 16

Methods of fresh tissue examination

Answer 16

1. Teasing or Dissociation
2. Squash preparation/Crushing
3. Smear preparation
4. Frozen section

Question 17

Immunofluorescence and immunoperoxidase techniques

Answer 17

1. Enzyme histochemistry
2. Electron Microscopy

Question 18

Alcohol solutions used as fixative

Answer 18

1. Methyl alcohol
2. Isopropyl alcohol (95%)
3. Ethyl alcohol

Question 19

Kinds of smear preparation

Answer 19

1. Streaking
2. Spreading
3. Pull apart
4. Touch preparation

Question 20

Aldehyde fixative

Answer 20

1. Formaldehyde
2. Glutaraldehyde

Question 21

Process of tissue preparation (IN ORDER)

Clue: F-D-C-I-E-T-S-S-M-L

Answer 21

1. Fixation
   2, Dehydration
2. Clearing
3. Impregnation
4. Embedding
5. Trimming
6. Section Cutting
7. Staining
8. Mounting
9. Labelling