Chapter 1: Aba Ginoong Maria (Remix) Flashcards
(21 cards)
Fixative recommended for center nervous tissue and general post-mortem tissues
10% Formal Saline
Excellent microanatomic fixative for pituitary gland
Zenker-Formol (Helly’s Solution)
Recommended mainly for tumor biopsies especially for the skin
Heidenhain’s Susa Solution
Recommended for demonstration of chromatin, mitochondria, mitotic figure, RBC’s and golgi bodies
Regard’s (Muller’s) Fluid
It demonstrates rickettsiea and other bacteria
Orth’s fluid
Recommended for acid mucopolysaccharides
Lead Fixatives
Fixes connective tissue mucin
Lead fixatives
Recommended fixation of embryos and pituitary biospies
Bouin’s Solution
Excellent fixative for glycogen
PICRIC ACID FIXATIVES
Brasil’s Alcoholic Picroformal Fixative
Considered to be the most rapid fixative
Carnoy’s fixative
Excellent fixative for nuclear structures (e.g chromosomes)
Flemming’s Solution
The fixative used at ice cold temperature ranging from -5 degrees celsius to 4 degree celsius
Acetone
The fixative contains mercuric chloride and glacial acetic acid
Zenker’s fluid
Commonly used for bone marrow biopsies
B-5 fixative
Cheap, readily available, easy to prepare, and widely used fixative
10% formal saline/formalin
Buffered formalin
Methods of fresh tissue examination
- Teasing or Dissociation
- Squash preparation/Crushing
- Smear preparation
- Frozen section
Immunofluorescence and immunoperoxidase techniques
- Enzyme histochemistry
- Electron Microscopy
Alcohol solutions used as fixative
- Methyl alcohol
- Isopropyl alcohol (95%)
- Ethyl alcohol
Kinds of smear preparation
- Streaking
- Spreading
- Pull apart
- Touch preparation
Aldehyde fixative
- Formaldehyde
- Glutaraldehyde
Process of tissue preparation (IN ORDER)
Clue: F-D-C-I-E-T-S-S-M-L
- Fixation
2, Dehydration - Clearing
- Impregnation
- Embedding
- Trimming
- Section Cutting
- Staining
- Mounting
- Labelling