Chapter 1: Cell Structure Flashcards
(44 cards)
Magnification
Image/Actual size
Magnification definition
how many times bigger an image of a specimen is observed is compared to the actual size of the specimen.
Light Microscope
Source: Light
Wavelength: 400-700nm
Resolution: 200nm
Specimen: Alive
Image: Coloured
Magnification: 1000-1500
Electron Microscope
Source: electron
Wavelength: 0.01nm
Resolution: 0.05nm
Specimen: Dead
Image: black and white
Transmission electron microscope (TEM)
the beam of electron is fired through the specimen.
Resolution as low as 0.05nm.
2D image
Scanning electron microscope (SEM)
the beam of electrons is fixed across the specimen.
Resolution of 3nm-20nm
3D image
Eye piece graticule
transparent scale used in the eye piece lens with 100 divisions with no dimensions
Stage micrometer
usually each division is 0.01mm
How do you calibrate an eyepiece graticule using a stage micrometer?
Place the stage micrometer on the microscope stage.
Align the stage micrometer scale with the eyepiece graticule.
Count how many divisions on the eyepiece graticule match a known length on the stage micrometer.
Calculate the value of one eyepiece division.
Resolution
The ability to distinguish between two closely spaced points as separate entities.
A wave length with high energy gives you a low or high resolution power?
Why?
High resolution power.
As you go up the elctromagnetic spectrum the energy increases
What does changing the objective lens changes?
The magnification
Not the resolution
Cell Surface Membrane
controls the movement of subtances on and out of the cell
Partially permeable
7nm in width
Formed from a bilayer of phospholipids
Acts like a barrier between the cytoplasm and the external environment
Site for enzyme-catalysed reactions
Nucleus
Largest organelle surrounded by a double mebraned nuclear envelope.
The nucleus contains DNA organized into chromosomes.
ribosomes production
Nuclear envelope
The nuclear envelope, a double membrane surrounding the nucleus, controls the exchange of materials (e.g., RNA and proteins) between the nucleus and cytoplasm
Nuclear pores
gaps in the nulcear envelope that allows exchange between the nucleus and cytoplasm
Nucleolus
manufacture ribosomes using the
information in its own DNA
Chromatin
the material of which chromosomes
are made
Cytoplasm
the contents of the cell excluding the nucleus
site for metabolic reactions in the cell
Rough endoplasmic recticulum (RER)
a network of membranous tubules and flattened sacs with ribosomes studded at its surface
Smooth Endoplasmic recticulum (SER)
synthesises lipids
Ribosomes
small, non-membrane-bound organelles responsible for protein synthesis.
20nm
70S ribosomes
found in:
Mitochondria
Chloroplast
80S ribosomes
Found in:
RER
Nucleus