Chapter 14 Flashcards by Megan Curts

Q

Amylase- Detects

A

Absence of Starch

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Q

Amylase Enzyme

A

Amylase

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Q

Amylase Substrate

A

Starch (Amylose)

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Q

Amylase Indicator

A

Iodine

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Q

Amylase Positive Result

A

Clear around growth-Glucose is present not starch, Amylase was produced.

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Q

Amylase Negative Result

A

Black Purple starch is present so no amylase.

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Q

Urease-Detects

A

Ammonia-Change pH

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Q

Urease Enzyme

A

Urease

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Q

Urease Substrate

A

Urea

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Q

Urease Indicator

A

Phenol Red

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Q

Urease Positive Result

A

Hot Pink

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Q

Urease Negative Result

A

Yellow Orange anything not hot pink

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Q

Catalase Detects

A

Oxygen

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Q

Catalase Enzyme

A

Catalase

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Q

Catalase Substrate

A

Hydrogen Peroxide

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Q

Catalase Indicator

A

Hydrogen Peroxide

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Q

Catalase Positive

Study These Flashcards

A

Bubbles

Q

Catalase Negative

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A

No Bubbles

Q

SIM Sulfur Detect

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A

Reduced Sulfer, Hydrogen Sulfide

Q

SIM Sulfur Enezyme

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A

Cysteine Desulfurase or Thiosulfate Reductase

Q

SIM Sulfur Substrate

Study These Flashcards

A

Cysteine or Sodium Thiosulfate

Q

SIM Sulfur Indicator

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A

Iron

Q

SIM Sulfur Positive

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A

Black Precipitate

Q

SIM Sulfur Negative

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A

No change in media

SIM Indole Detect

Indole

SIM Indole Enzyme

Tryptophanase

SIM Indole Substrate

Tyroptophan

SIM Indole Indicator

Kovacs

SIM Indole Positive Result

Red color at top

SIM Indole Negative Result

No change in media

SIM Motility Detect

Movement of bacteria away from line where needle inoculated the media

SIM Motility Enzyme

None

SIM Motility Substrate

None

SIM Motility Indicator

None

SIM Motility Positive

Growth away from stab line

SIM Motility Negative

No growth at stab line

Citrate Detect

Citrate utilization as a Carbon source

Citrate Enzyme

Citrate Permease, Citrase

Citrate Substrate

Citrate

Citrate Indicator

Bromthymol Blue

Citrate Positive

Blue

Citrate Negative

Forest Green