Chapter 15: Toxicogenomics

Question 1

What does the increase VS decrease in the concentration of transcription mean?

Answer 1

decrease [transcription]= not a lot of protein being made
increase [transcription]= a lot of protein being made

Question 2

How does DEET exposure affect dog ticks?

Answer 2

Found that exposure to DEET causes dog ticks to rapidly decrease expression of Phase I detoxification genes!
Also found decreased P450 enzyme activity!

Question 3

PCBs and Dioxins bind to what receptor? What happens?

Answer 3

PCBs and Dioxins bind to the aryl hydrocarbon receptor

This is a transcription factor protein. Once it gets activated, moves into the nucleus and switches on the transcription of 60 genes

Question 4

What are the two components of lampricide? and do they specify?

Answer 4

TFM (lamprey specific) and Niclosamide (no specificity)

Question 5

How does TFM work?

Answer 5

It interferes with oxidative phosphorylation. inhibits ATP synthesis, leads to ROS production and necrosis

Question 6

Why can the [lactate] be used as a biomarker to indicate if the organism is okay or not?

Answer 6

↑[lactate]= not enough O2. fermentation is happening
↓[lactate]= enough O2