Chapter 17

Question 1

What genera make up the family Neisseriaceae?

Answer 1

Neisseria
Kingella,
Eikenella,
Simonsiella,
Alysiella, and several other genera.

Question 2

General characteristics of Neisseria spp.

Answer 2

mbost Neisseria spp. are
aerobic,
nonmotile,
non- spore-forming
gram-negative diplococci.

Question 3

Which Neisseria spp are are rod shaped

Answer 3

Neisseria elongata, Neisseria weaveri, and Neisseria bacilliformis are known exceptions and are rod shaped.

Question 4

All Neisseria species are cytochrome oxidase and catalase positive except for

Answer 4

N. elongata subsp. nitroreducens and N. bacilliformis, which are catalase negative.

Question 5

Many Neisseria spp. are capnophilic? T/F?

Answer 5

T

Question 6

Can Neisseria spp. grow anaerobically and how?

Answer 6

Yes. If the alternative electron acceptors are available such as Nitrites.

Question 7

Where are the natural habitats of Neisseria spp.

Answer 7

mucous membranes of the respiratory and urogenital tracts

Question 8

Neisseria gonorrhea is also known as

Answer 8

GonoCOCCI

Question 9

Neisseria meningitidis is also known as

Answer 9

MeningoCOCCI

Question 10

All Neisseria genera are oxidase positive?

Answer 10

True!

Question 11

All Neisseria genera are catalase negative except

Answer 11

Neisseria

Question 12

Which species of Neisseria are pathogenic

Answer 12

Neisseria gonorrhoeae
Neisseria meningitidis
*Neisseria weaveri (bacillus)

Question 13

Which species of Neisseria is normal oral flora biota of dogs and can be found in bite wounds.

Answer 13

Neisseria weaveri (bacillus)

Question 14

morphologically and biochemically similar to the Neisseria spp. but is NOT a member of the family Neisseriaceae

Answer 14

M. catarrhalis

Question 15

Growth requirements for N. gonorrhoea and N. meningitidis?

Answer 15

Both N. gonorrhoeae and N. meningitidis require iron for growth.
They compete with their human host by binding human transferrin to specific surface receptors.
OF NOTE:

Transferrin is a blood plasma glycoprotein that plays a central role in iron metabolism and is responsible for ferric-ion delivery. Transferrin functions as the most critical ferric pool in the body. It transports iron through the blood to various tissues, such as the liver, spleen, and bone marrow.

Question 16

Commensalis in the upper RT of dogs

Answer 16

N. weaveri

Question 17

Virulence factors of Neisseria

Answer 17

• Receptors for human transferrin
• Capsule (N. meningitidis)
• Pili (fimbriae)
• Cell membrane proteins
• Endotoxin
• Lipooligosaccharide (LOS)
• IgA protease

Question 18

What differentiates N. gonorrhea in the 5 morphologically distinctive colony types T1-T5

Answer 18

The presence or absence of Pili.
* fine hairlike projections that are important in:
- The initial attachment of the organism to host tissues.
- Inhibits phagocytosis of organism by neutrophils
- Aids in exchange of genetic material between cells

Question 19

Which colony types of N. gonorrhea a virulent. Do they possess Pili?

Answer 19

T1-T2. They possess PILI AND ARE VIRULENT!

Question 20

Which N. gonorrhea colony type are avirulent and do they possess Pili?

Answer 20

T3-T5 and they DO NOT HAVE PILI!

Question 21

Gonorrhea infection is _________ and which cells it affects

Answer 21

Acute pyogenic
NON-Ciliated COLUMNAR AND TRANSITIONAL EPITHELIUM

Question 22

Gonorrhea incubation period

Answer 22

2-7 days

Question 23

Protein I function and other name

Answer 23

Porin protein (Por)
Nutrient and waste exchange via channels

Question 24

Genes that encode Por protein

Answer 24

porA and porB

Question 25

Which Neisseria species encodes both porA and porB genes

Answer 25

N. meningitidis

Question 26

Which Neisseria species expresses only porB genes

Answer 26

N. gonorrhea

Question 27

What other function does porB serve

Answer 27

Protective against the hosts inflammatory response and serum complement-mediated killing.

Question 28

Symptoms of gonorrhea in men

Answer 28

Acute urethritis that results in purulent discharge and dysuria. Complications include prostatitis and epididymitis.

Question 29

Most common site of gonorrhea infection in women

Answer 29

ENDOCERVIX

Question 30

Symptoms of gonorrhea infection in women

Answer 30

Dysuria, cervical discharge and lower abdominal pain. Complications for untreated infection: PID which may cause sterility, ectopic pregnancy or perihepatitis (FITZ-HUGH-CURTIS Syndrome)

Question 31

Blood borne dissemination of N. gonorrhea results in

Answer 31

Purulent arthritis

Question 32

Most disseminated gonorrhea infections are found in males or females? And a due to which strain?

Answer 32

Females. Arginine Hypoxanthine Uracil strain (AHU).

Question 33

Which anticoagulant inhibits N. gonorrhea

Answer 33

Sodium Polyanethol Sulfonate (SPS) * add gelatin to neutralize inhibitory effects of SPS

Question 34

N. gonorrhea in newborns is called

Answer 34

OPTHALMIA NEONATORUM. Can result in blindness if untreated

Question 35

What antibiotic eye drops placed in newborn eyes to prevent N. gonorrhea

Answer 35

Erythromycin

Question 36

N. gonorrhea specimen of choice in men and women

Answer 36

Men: urethra Women: endocervix

Question 37

What type of swabs are inhibitory to gonorrhea

Answer 37

Calcium alignate & cotton