CHAPTER 2

Question 1

During replication, the DNA synthesis can only make one direction which is 3’ to 5’ direction

Answer 1

False

Question 2

DNA replication results in 2 DNA molecules, 1 with two new strands and the other with two original strands

Answer 2

False

Question 3

The DNA under the control of the origin of replication i

Answer 3

Replicon

Question 4

DNA replication is carried out in all organisms by a multiprotein complex called

Answer 4

Replisome

Question 5

It is a type of DNA damage, that has a cross-links adjacent pyrimidine on the same DNA strand, forming 2 major lesions, the vast majority of the lesions are pyrimidine dimers in which the 2 carbons on each of the two pyrimidine form a cyclobutyl ring

Answer 5

UV Radiation

Question 6

The replication forks move bidirectionally, and they keep moving until they reach the end of the chromosome, which is known as

Answer 6

Telomere

Question 7

If an alpha catalytic subunits continues to synthesize in a short stretch of DNA before the DNA polymerase takes over the replucation process, this will be called as

Answer 7

Polymerase Switching

Question 8

In a base excision repair, a base that has been damaged by oxidation or chemical modification can be recognized by

Answer 8

DNA glycosylase

Question 9

Complementary strand for the DNA sequence 5’-GATCCTGAAC-3’

Answer 9

3’-CTAGGACTTG-5’

Question 10

During the replication process, what variety of DNA hypothesis does the DNA replication mechanism occurs?

Answer 10

Semiconservative model

Question 11

Valid on the process of DNA replication

Answer 11

DNA gyrase and helicase work to unwind the DNA so that it can be replicated

Question 12

In the process of DNA replication, both replication forks will travel in the same direction and helicase will work to unwind the DNA, allowing the synthesis of new strands to occur

Answer 12

False

Question 13

A _ is a sequence involved in termination, that contains a short 23-bp sequence

Answer 13

Ter site

Question 14

An enzyme that can only add to nucleotides to the 3’ end of a nucleotide fragment?

Answer 14

DNA polymerase

Question 15

An enzyme that harness the chemical energy for the ATP hydrolysis to separate the 2 DNA strands at the replication fork

Answer 15

Helicase

Question 16

In a homologous recombination, the process occurs randomly around a chromosome, and when an area of chromosome shows recombination, they are called the hot spots

Answer 16

False

Question 17

A type of protein that is associated with the clamp loader and links to the polymerase-clamp loader complex to the helicase

Answer 17

Tau

Question 18

James Watson and Francis Crick discover the structure of DNA using

Answer 18

X-ray Crystallography

Question 19

If each cell needs a faithful set of genetic materials, then a parental cell must duplicate its entire genome before diving it. Afterward, each of the 2 new daughter cells that were created will then receive 1 copy of the genome through a process of cell division. Thus, DNA replication is a process that ensures that the DNA is duplicated before cell division

Answer 19

True

Question 20

It is a two band-gradient that after 1 generation, 1 band will be representing a (15N/15N) parental duplex, plus a (14N/14N) newly synthesized DNA

Answer 20

Conservative mechanism