chapter 2: tools of the lab

Question 1

what is a microscope?

Answer 1

a scientific tool that lets us see things that are normally too small to see with the naked human eye

Question 2

where are the objective lenses on the microscope?

Answer 2

closer to the specimen

Question 3

where are the ocular lenses on the microscope?

Answer 3

the ones we use at our eyes

Question 4

what do we multiply to get the magnification ?

Answer 4

we multiple the ocular by the objective

Question 5

true/false : the image we see with the microscope is in reverse

Answer 5

true

Question 6

what does it mean when we have regulation of an image?

Answer 6

it means that we have a clearer picture

Question 7

the resolving power of the human eye is ___ mm and that of a microscope with oil immersion is ___ μm

Answer 7

0.2, 0.2

Question 8

how does the lens of the microscope function?

Answer 8

it refracts light

Question 9

describe bright field microscopy

Answer 9

when the background is light of the specimen but the specimen itself is dark

Question 10

what is dark field microscopy?

Answer 10

when the background is dark, but the specimen is bright

Question 11

true/false : phase contrast microscopy is good for living samples

Answer 11

true

Question 12

are stains involved in fluorescence microscopy ?

Answer 12

yes

Question 13

what is a use of flourescent microscopes?

Answer 13

seeing individual cell structures, examining structures

Question 14

what is electron microscopy?

Answer 14

when electrons are emitted for a light source instead of a light

Question 15

what is heat fixation?

Answer 15

burning a copy of the sample on a plate to look at it under the microscope

Question 16

what are the components to a stain?

Answer 16

a dye and a binding stain

Question 17

what is the difference between positive and negative staining?

Answer 17

positive means it sticks on the sample, negative does not stick

Question 18

which is more complicated, a simple or differential stain?

Answer 18

a differential stain because of the more components inside the ink

Question 19

what are the 2 types of stains in the gram stain?

Answer 19

gram positive and negative

Question 20

true/false : the colors for gram stains are pink and purple

Answer 20

true

Question 21

does the endospore stain use heat for its stain process?

Answer 21

yes

Question 22

what is a capsule stain used for?

Answer 22

to see stains that are not permeable with dye

Question 23

what is flagella staining?

Answer 23

staining to see movement in cells

Question 24

what is inoculation?

Answer 24

when you take a sample to put on a growth medium

Question 25

what is incubation?

Answer 25

when we put the sample in an environment that mimics the natural habitat that it would have experienced

Question 26

what are the 2 forms of agar?

Answer 26

solid and liquid

Question 27

what is agar extracted from?

Answer 27

algae

Question 28

what is defined media ?

Answer 28

when all components of the sample are known

Question 29

what is enriched media?

Answer 29

media that has additives in it to encourage growth

Question 30

what is differential media?

Answer 30

media that distinguished between groups of microbes

Question 31

true/false : media can be of only 1 type

Answer 31

false

Question 32

what does transport media do?

Answer 32

guarantee that all media is safely transported from one area to another

Question 33

what is a colony

Answer 33

one section of microorganisms that are macroscopically different from another

Question 34

know how to perform a streak plate method of culture