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Microbiology > Chapter 20- Neisseriae > Flashcards

Chapter 20- Neisseriae Flashcards

(54 cards)

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1
Q

human pathogen that is associated with or inside polymorphonuclear cells

A

Neisseria meningitidis (meningococci)

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2
Q

Gram-negative, nonmotile diplococcus, 0.8 um kidney-bean shaped

A

Typical Neisseria

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3
Q

In what hour does gonococci and meningicocci form convex, glistening, elevated mucoid 1-5 mm in diameter

A

48 hours

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4
Q

Describe colonies of a typical Neisseria

A

Transparent or opaque, nonpigmented and non-hemolytic

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5
Q

Nesseria species that produces yellow pigment

A

N.flavescens
N. subflava
N. cineria
N. lactamica

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6
Q

Nesseria sp that produces opaque, brittle, wrinkled colonies

A

N.sicca

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7
Q

produces nonpigmented or pinkish gray opaque colonies

A

Moraxella catarrhalis

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8
Q

Nesseria species that oxidizes glucose

A 
N. gonorrhea 
N. meningitidis
N. lactamica
N. sicca
N. subflava
N. mucosa
N. polysaccharea
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9
Q

Nesseria species that oxidizes maltose

A 
N. meningitidis 
N. lactamica
N. sicca
N. subflava
N. mucosa
N. polysaccharea
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10
Q

Nesseria species that oxidizes lactose

A

N. lactamica

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11
Q

Nesseria species that oxidizes sucrose

A

N. sicca
N. subflava
N. mucosa

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12
Q

Nesseria species that has DNAse

A

Moraxella catarrhalis

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13
Q

Media where meningococci and gonococci grows best

A 
On organic substances 
Heated blood
Hemin
Animal proteins
5% CO2
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14
Q

Growth of meningococci and gonococci is inhibited by

A

Fatty acids and salts

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15
Q

meningococci and gonococci are rapidly killed by

A

drying
sunlight
moist heat
disinfectants

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16
Q

Gonococci require these products in order to grow

A

Arginine
Hypoxanthine
Uracil

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17
Q

Colonies of gonococci in selective subculture

A

Small colonies with piliated bacteria

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18
Q

Colonies of gonococci in nonselective subculture

A

Larger colonies with non-piliated gonococci

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19
Q

Antigenic structure of N.gonorrhea

A
  1. Pili (Fimbrae)
  2. Por
  3. Opa proteins
  4. Rmp (Protein III)
  5. Lipooligosaccharide
  6. Lip (H8)
  7. Fbp (ferric-binding protein)
  8. IgA1 protease
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20
Q

Antigenic structure of N.gonorrhea that enhance attachment to host cells and resistance to phagocytosis

A

Pili (fimbriae)

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21
Q

Antigenic structure of N.gonorrhea that prevents phagosome-lysosome fusion

A

Por proteins

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22
Q

Antigenic structure of N.gonorrhea that function in adhesion within colonies and their attachment to host cell receptors

23
Q

Antigenic structure of N.gonorrhea that is associated with Por in the formation of pores in the cell surface

Study These Flashcards
A

Rmp (Protein III)

24
Q

Antigenic structure of N.gonorrhea that helps them evade immune recognition of the host cell

Study These Flashcards
A

Lipooligosaccharide

25
Antigenic structure of N.gonorrhea that is a surface-exposed protein that is heat modifiable like Opa.
Lip (H8)
26
Antigenic structure of N.gonorrhea that is similar in MW to Por that is expressed when the iron supply is limited
Ferric-binding protein (Fbp)
27
Antigenic structure of N.gonorrhea that splits and inactivates IgA1
IgA1 protease
28
Por proteins selectively bind to what complement
C3b and C4b
29
Opaque gonococci colonies is associated with?
Men with SYMPTOMATIC urethritis | Uterine cervical cultures at midcycle
30
Transparent gonococci colonies is associated with
ASYMPTOMATIC urethral infection in men Menstruating women Patients with invasive form of gonorrhea
31
Treatment prevention for gonococcal ophthalmia neonatarum (SET)
silver nitrate erythromycin tetracycline
32
Specimes for diagnostic lab tests for N.gonorrheae
Pus Secretions Synovial fluid Blood culture
33
Tests for gonococcal smears in women
NAATs or cultures
34
Media best for gonococci
Modified Thayer-Martin medium (MTM) 5% CO2 at 37 C
35
To avoid overgrowth of gonococci, selective medium must contain (T-VAC)
Trimethoprim (3 ug/mL) Vancomycin (3 ug/mL) Amphotericin B (1 ug/mL) Colistin (7.5 ug/mL)
36
_____ hours after culture, gonococci that can identified by gram-stained smear
48 hours
37
Gonococci can be identified through
Oxidase positive Coagglutination Immunofloresence
38
Test for direct detection of N.gonorrhea
NAATs
39
Antibodies present against gonococcal pili, outer membrane proteins and LPS
IgA IgG IgM
40
Antibodies against gonococcal pili, and outer membrane proteins are detected by
Immunoblotting Radioimmunoassay ELISA
41
Treatment for gonococcal infections
IM ceftriaxone 250 mg | Oral cefixime 400 mg
42
Treatment for gonococcal infections with concomitant chlamydial infections
1 g Azithromycin or | 100 mg of Doxycycline oral for 7 days
43
Treatment for gonococcal ophthalmia neonatarum
0.5% erythromycin ophthalmic ointment | 1 % tetracycline ointment for conjunctiva
44
Most important serogroups of N.meningitidis that are associated with human disease
A, B, C, X, Y and W-135
45
Meningococcal antigens are found in
Blood and CSF
46
The outer membrane of N.meningitidis contains these things that plays a role in their virulence
Proteins and LPS
47
Responsible for toxic effects of meningococcal disease
Lipid A disaccharide of meningococcal LPS
48
Portal on entry of N.meningitidis
Nasopharynx
49
Meningococci are readily phagocytosed in the presence of
Specific opsonin
50
Specimens used for diagnostic lab tests for meningococci
NP swab culture | Puncture material from petechiae
51
Culture media for N. meningitidis
CSF on chocolate agar at 37 C 5% CO2
52
Drug of choice for N. meningitidis
Pen G
53
Drug for N. meningitidis that is allergic to Pen G
Chloramphenicol | 3rd Gen Cephalosporins (Cefotaxime or ceftriaxone
54
M.catarrhalis produces this enzyme that forms the basis for rapid fluorometric tests
Butyrate esterase

Microbiology (7 decks)

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