Chapter 8

Question 1

RNA polymerases

Eukaryotic RNA

Answer 1

RNA Pol 1-rRNA
RNA Pol 2-mRNA
RNA Pol 3-tRNA
RNA pol 4-siRNA

Question 2

Bacteria RNA polymerase

Answer 2

Single type of RNA polymerase (alpha Beta Omega)

Alpha subunits-composed of an N terminal domain (alpha NTD) C terminal domain (alphaCTD) [act as hinges]

Question 3

Archaeal RNA polymerase

Answer 3

Single type of RNA pol

More complicated more subunits then bacterial RNA polymerase

Question 4

Eukaryotic RNA polymerases

Answer 4

Multiple RNA polymerase
Tertiary polymerase
Contain subunits for the core enzyme and require additional proteins to bind and recognize to promoters

Question 5

Nucleosome remodeling enzymes

Answer 5

Make DNA accessible for transcription

Reposition histone and histone octamer’s from one location of the DNA to another

Question 6

RNA polymerase 2

Answer 6

Two functions:
Transcription
RNA processing-facilitates the addition of guanocine cap to the 5’ and is involved in adding poly A tail

Question 7

Making the DNA accessible for transcription (3)

Answer 7

Nucleosome remodeling enzymes
Histone chaperones
Histone modifying enzymes