Chapter 8: DNA Technology Flashcards
(5 cards)
1
Q
Restriction enzymes
A
Cut DNA based on target sequence
2
Q
DNA Ligase
A
Enzyme
Seals or lighted DNA pieces together
3
Q
Making Recombinant DNA by use of R. Enzyme and Ligase
A
- restriction enzyme cuts DNA
- addition of DNA fragment from another source (stick by base pairing)
- fragment produced by same restriction enzyme
- one possible combo
- DNA ligase seals strand
4
Q
Cloning
A
- isolate gene of interest
- cut vector with R.E. for compatible strands
- Mix2 DNA’s together, ligate
- transform
- screen
5
Q
Goals
A
Eliminate undesirable phenotypic traits
Combine beneficial traits to make new organisms
Create organisms that synthesize product for human needs
