Chapter 8 Gene Mutations

Question 1

What are the stop codons?

Question 2

What is a silent mutation

Answer 2

A mutation that does not change the Amino acid sequence

Question 3

What is a nonsense mutation?

Answer 3

A mutation that makes a stop codon, prematurely truncating it.

Question 4

How does horseradish peroxidase work?

Answer 4

Bind 2ndary ab to target. Horseradish peroxidase cleaves the substrate that came with kit. When cleaved color is released where the ab binded to.

Question 5

What is HPLC

Answer 5

High performance liquid chromatography

Question 6

SSCP

Answer 6

Single strand conformation polymorphism. Looks at how the sequence folds which ends up affecting their ability to migrate through a gel. Since bp mutations will change the conformation.

Question 7

Allele specific hybridization
Allele specific oligomer

Answer 7

ASO

Question 8

RFLP

Answer 8

Restriction fragment length polymorphism. Cut the amplicon region with restriction enzymes. Normal should have 1 band, mutates will have more than 1. A heterozygous will have the normal and mutated result