Chapters 3 And 7 Flashcards

(90 cards)

1
Q

Who proposed the concept of spontaneous generation?

A

Aristotle (384-322 BCE)

Living things can come from nonliving things. This concept is known as spontaneous generation < false!!

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2
Q

What did Jean Baptist Van Helmont believe mice came from?

A

Dirty rags/ rotting grains

mice come from dirty rags/ rotting grains

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3
Q

Who was the first to describe cells based upon his microscopic observations of cork?

A

Robert Hooke

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4
Q

What theory did Robert Hooke contribute to?

A

Cell theory

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5
Q

What are Monosaccharides?

A

3- to 7-carbon sugars. Examples : Glucose, fructose. Sugars involved in metabolic reactions; building block of disaccharides and polysaccharides

Monosaccharides are single sugar units.

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6
Q

What are Disaccharides?

A

Two monosaccharides. Example :Maltose(maltsugar), Lactose (milk sugar), Sucrose (table sugar). Composed of two glucoses; an important breakdown product of starch

Disaccharides are composed of two sugar units.

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7
Q

What are Polysaccharides?

A

Chains of monosaccharides.
Examples : Starch, cellulose, glycogen.

Major component of cell membranes and storage

Polysaccharides are long chains of sugar units.

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8
Q

What are 3 main types of Lipids?

A

Triglycerides, Phospholipids, Sterols

Lipids are a diverse group of molecules including fats and oils.

An example would be triacylglycerol

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9
Q

What is a primary protein structure?

A

A sequence of a chain of amino acids

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10
Q

What is a secondary protein structure?

A

folding of the polypeptide chain into (alpha) helices or (beta) sheets

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11
Q

What is a tertiary protein structure?

A

A three-dimensional folding pattern of a protein due to side chain interactions

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12
Q

What is a quaternary protein structure.

A

protein consisting of more than one amino acid chain

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13
Q

How do we read a sequence of amino acids?

A

From the N terminus to the C terminus

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14
Q

What is the Meat Maggot Experiment?

A

Observation: There are flies in a butcher shop

Question: Does rotting meat turn into or produce the flies?

Hypothesis: Rotten meat does not turn into flies. Only flies can make more flies.

Prediction: If meat cannot turn into flies, rotting meat in a sealed (fly-proof) container should not produce flies or maggots.

Test:
Control jars - Flies enter jars. Later, maggots, then more flies seen on meat.

Gauze-covered jars - No maggots or flies were ever seen on the meat even though flies were on gauze.

open container
cork-sealed container
gauze-covered container

Sealed jars - No maggots or flies were ever seen on the meat.

Conclusion(s): Maggots arose only where flies were able to lay eggs directly on the meat. Thus, only flies can make more flies. This experiment disproved the idea of spontaneous generation for larger organisms only.

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15
Q

Who did the Meat Maggot experiment?

A

Francesco Redi

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16
Q

What are Robert Koch’s 4 postulates?

A
  1. The microorganism must be found in abundance in all organisms suffering from the disease, but should not be found in healthy organisms.
  2. The microorganism must be isolated from a diseased organism and grown in pure culture.
  3. The cultured microorganism should cause disease when introduced into a healthy organism.
  4. The microorganism must be reisolated from the inoculated, diseased experimental host and identified as being identical to the original
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17
Q

What French scientist used a swan-neck feature of the flasks where it allowed air to enter the flask but prevented the entry of bacterial spores?

A

Louis Pasteur

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18
Q

What are cytosine and thymine
A. Purines
B. Pyrimidines
C. Both
D. Neither

A

B. Pyrimidines

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19
Q

What 2 are considered purines?
A. Adenine and cytosine
B. Guanine and thymine
C. Cytosine and adenine
D. Adenine and guanine

A

D. Adenine and guanine

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20
Q

True or false :

DNA is a double stranded molecule while RNA is single stranded

A

True

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21
Q

What number structure of a protein may be an alpha-helix or a beta-pleated sheet or both?

A

Secondary structure of a protein

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22
Q

What are the 3 levels of 3-D protein folding?

A

Primary, secondary, and tertiary

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23
Q

What color are gram-negative bacteria in a gram stain?

A

Pink

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24
Q

What color are gram-positive bacteria after gram staining?

A

Purple

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25
What are monomers?
Amino acids
26
What are polymers?
Peptides / proteins
27
Glucose and fructose combine to make what molecule and with what kind of bond between them?
Sucrose ; Glycosidic bond
28
What kind of prokaryote has a round sphere shape?
Coccus
29
What kind of prokaryote has a long oval Rod shape?
Bacillus
30
What kind of prokaryote has a curved rod, oval shape?
Vibrio
31
What kind of prokaryote has a small rod, oval shape?
Coccobacillus
32
What kind of prokaryote has a wavy spiral shape?
Spirillum
33
What kind of prokaryote has a long helical spiral shape?
Spirochete
34
Who founded Agar after learning about it from their neighbor from Indonesia, and told Robert Koch to use for his experiments?
Angelina Fanny Hese
35
What pH is considered acidic?
0-6
36
What pH is considered basic?
8-14
37
What pH is considered neutral?
7
38
What are the 5 properties of water?
1. Polar 2. High specific heat (amount of energy is takes to go up 1 degree) 3. Adhesion (water to glass) and cohesion (water to water) 4. High surface tension 5. A good solvent (forms ions)
39
What is a catabolic reaction?
Breaks things down (cata - cuts) and gives off energy Aka : decomposition
40
What is an anabolic reaction?
Adds things together using energy Aka : Synthesis
41
Which domain has the most extremophiles? A. Domain bacteria B. Domain archaea C. Both D. Neither
B. Domain Archaea
42
What 4 characteristics do all cells have?
Plasma membrane Ribosomes DNA Cytoplasm
43
What is a pair of 2 cocci called?
Diplococcus
44
What is a group of 4 cells arranged in a square called?
Tetrad
45
What is a chain of cocci called?
Streptococcus
46
What is a chain of cocci called?
Streptococcus
47
What is a cluster of cocci called?
Staphylococcus
48
What is a chain of rods called?
Streptobacillus
49
What 2 alternating molecules make up bacterial cell walls?
NAG and NAM
50
What 2 alternating molecules make up bacterial cell walls?
NAG and NAM
51
What do alternating subunits of NAM and NAG cross-linked via their tetrapeptide chains create?
Peptidoglycan
52
Do gram-positive or gram-negative bacteria have a thick layer of peptidoglycan?
Gram-positive bacteria
53
Do gram-positive or gram-negative bacteria have a thin layer of peptidoglycan?
Gram-negative bacteria
54
What type of gram-positive bacteria has waxy mycolic acids?
Gram-positive acid-fast bacteria
55
Do gram-positive or gram-negative bacteria have lipopolysaccharide in the outer membrane?
Gram-negative
56
What are porins ?
Porins are where the phages bind to
57
What interferes with peptidoglycan synthesis? A. Penicillin B. Lysozyme C. Lipopolysaccharide D. Iodine
A. Penicillin
58
What breaks tetrapeptide bonds linking glycan chains? A. Penicillin B. Lipopolysaccharide C. Lysozyme D. None of the above
C. Lysozyme
59
What are the 4 steps of gram staining?
1. Crystal violet - stains cells purple or blue 2. Iodine - makes dye less soluble so it can stick to cell wall 3. Alcohol - decolorizer that washes away stain from gram-negative walls 4. Safranin - a counter stain that allows dye to stick to gram-negative cells (makes pink)
60
True or false : Do most bacteria have a cell wall?
True
61
What 2 layers help bacteria avoid phagocytosis?
Slime layer - diffuse, irregular Capsule layer - distinct, gelatinous
62
What is pili used to exchange?
Used to exchange genetic material
63
What is fimbriae used for on a bacterial cell?
helps bacterial cell to adhere to surfaces
64
What are the 3 parts of the flagellar anatomy?
1. Filament 2. Hook 3. Basal body
65
What type of flagellated bacteria has only 1 flagella on one end?
Monotrichous
66
What type of flagellated bacteria has 1 on each end (2 total)?
Amphitrichous
67
What type of flagellated bacteria has multiple flagella on one end only?
Lophotrichous
68
What type of flagellated bacteria has lots of flagella all over?
Peritrichous
69
What are the motions a flagella does?
Tumbling (clockwise rotation of flagella) and running (counter-clockwise rotation of flagella)
70
Do prokaryotes or eukaryotes have ribosome subunits: 70S, 50S, and 30S?
Prokaryotes
71
Do prokaryotes or eukaryotes have ribosome subunits: 80S, 60S, and 40S
Eukaryotes
72
What is metabolism?
Taking nutrients into the cell and converting them into cell parts and energy
73
What is the loss of electrons?
Oxidation
74
What is the gain of electrons?
Reduction
75
What decreases the activation energy?
Enzymes
76
What do catalysts do?
Speed up rate of reaction
77
What enzyme likes it at 37C?
Mesophile
78
What enzyme has growth at 65C?
Thermophile
79
What enzyme works best at a pH of 7?
Neutrophile
80
What enzyme works the best at pH 3.
Acidophile
81
what 2 things can pyruvate turn into with fermentation?
ethyl alcohol or lactic acid
82
where does glycolysis happen in the cell?
cytoplasm
83
true or false glycolysis happens in both anaerobic and aerobic organisms
true
84
what 2 pathways does aerobic respiration go through?
krebs cycle and oxidative phosphorylation(ETC)
85
how many ATP does 1 NADH make?
3 ATP 1 NADH = 3 ATP
86
how many ATP does 1 FADH2 make?
2 ATP 1 FADH2 = 2 ATP
87
how many total ATP does Aerobic respiration make?
38 total ATP (36-38)
88
what are the 4 steps of aerobic respiration?
1. Glycolysis 2. prep step 3. Krebs cycle 4. Electron transport chain
89
what 2 cycles happen for anaerobic respiration?
Krebs cycle and ETC
90
what is Protein Hydrolysis?
cutting the peptide bond to just amino acids