Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Microbio Lab Practical > Characteristic Tests > Flashcards

Characteristic Tests Flashcards

1
Q

Glucose: color change

A

Red to Yellow if fermentation is present

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Glucose: pH indicator

A

Phenol Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Glucose Fermentation Process

A

(1) Fermentation positive

(2) They release acidic byproducts (3) Leading to a color change

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Lactose: color change

A

Red to Yellow if fermentation is present

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Lactose: pH indicator

A

Phenol Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Lactose Fermentation Process

A

(1) Fermentation (+)
(2) Acidic byproduct
(3) Color change

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Sucrose: color change

A

Red to Yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Sucrose: pH indicator

A

Phenol red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Sucrose: Fermentation Process

A

(1) Fermentation (+)
(2) Acidic by product
(3) Color change

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Mannitol: color change

A

Red to Yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Mannitol: pH indicator

A

Phenol red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Mannitol: Fermentation Process

A

(1) Fermentation (+)
(2) Acidic by product
(3) Color change

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Tryptone: color change

A

Clear to Clear with pink ring

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Tyrptone: Reagent

A

Kovacs reagent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Tryptone: Production pathway

A

Tryptophan (AA) in media breaks down into indole and pyruvic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

MR-VP: color change

A

Clear to clear/growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

MR: what type of pathway

A

Mixed Acid Fermentation Pathway

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

MR: test for positive and negative

A

(1) Straight line of diffused color = positive

(2) Diffused color = negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

VP: what type of test

A

2,3 butanediol production pathway

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Urea: color change

A

Yellow/orange to light/hot pink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Urea: Purpose

A

Check for urease enzyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Urea: pH indicator

A

Phenol Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Urea: production pathway

A

(1) Urea
(2) To ammonia
(3) pH increases
(4) Turns pink in color

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Simmons citrate: color

A

(1) Green to blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Simmons citrate: Purpose
To see if organism can use citrate for growth/ metabolism
26
Simmons citrate: pH indicator
Bromothymol blue
27
Simmons citrate: Production pathway
Citrate to Pyruvic acid
28
Phenylalanine slant: color change
Clear to Green
29
Phenylalanine slant: Purpose
Check for presence of phenylalanine deaminase enzyme
30
Phenylalanine slant: Reagent
Ferric chloride
31
Phenylalanine slant: Production pathway
(1) Phenyl pyruvic acid and ammonia by product from Phenylalanine AA
32
Kligers Iron: color change
Red to Red with a black butt
33
Kligers Iron: Purpose
Check for presence of Cysteine desulfurase enzyme
34
Kligers Iron: Production pathway
Cysteine to H2S and Iron leading to a black butt which is anaerobic
35
Semi solid agar: color change
Clear to clear with red streak line | (1) colorless in oxidized state (2) red in reduced state
36
Semi solid agar: Purpose
Check for motility
37
Semi Solid Agar: Checking for what presence
TTC
38
Nutrient Agar: color
Clear to clear with colony growths
39
Eosin methylene blue
(1) Purple/red plate to purpleish pink colony growths (2) Green if colony growths/ fermentation is positive (3) Checks for fermentation of lactose (4) pH sensitive dye is methylene blue (5) Selective for gram (-) organisms
40
Mannitol Salt Agar Plate
(1) Check for mannitol fermentation (2) pH sensitive dye: phenol red (3) If fermentation is positive the plate turns yellow, if not it turns pink (4) Selects for organisms that can handle high salt concentrations
41
Blood Agar Plate
Used for throat culture because the blood agar breaks down hemoglobin to get iron
42
TSA Plate
Universal Plate
43
Partial Lysis Blood cells
Streptococcal pneumoniae (green pigment)
44
Complete Lysis Blood cells
Streptococcal pyrogenes (clear spots)
45
No lysis Blood cells
Enterobacter faecalis
46
MH plate
Mueller Hinton, Universal Plate
47
Brewers Plate
Contains sodium thioglycollate
48
Resazurin
Oxygen indicator in FTG tube and Brewers plate
49
Agar Deep
Check to see if organism is anaerobic, aerobic, facultative anaerobic, aero-tolerant anaerobe
50
FTG Tube
(1) Fluid thioglycollate, contains sodium thioglycollate, converts O2 to H2O (2) Favors anaerobic
51
Facultative Anaerobic
Favors aerobic conditions but will still grow anaerobically
52
Obligate Anaerobe
Only grows without oxygen
53
Aerobe
Only grows with oxygen
54
Aerotolerant Anaerobe
Grows with out without oxygen evenly
55
UV rays
Forms pyrimidine dimers to kill organisms (1) 260 nm is most germicidal effect (2) 4nm UV (3) 800 nm is infrared
56
Quadrant streaking
Check for contamination
57
Grams Iodine and Crystal violet
Forms an insoluble complex in the PG layer in gram +
58
Catalase
(1) Purpose: catalase (2) Hydrogen peroxide to H2O and O2 (3) Bubbles
59
Gram positive characterisitics
Purple, clustered
60
Gram negative characteristics
Pink, linked
61
Sabouraud Agar
Low pH so good for skin growing organisms
62
Antiseptic
can be used on living tissue
63
Disinfectant
can be used on inanimate objects
64
Antigen
A foreign substance that enters the body and mounts an immune response
65
Antigen binding site
Protein sequence, receptor, epitope
66
Antibody
Response of human immune system to any foreign substance is to produce anti- antigen, antibody it is also referred to as immune response
67
Immunity Innate vs Acquired
(1) Innate is born with it its natural (2) Acquired is from mother or from vaccine
68
Type A
Antibody B, Antigen A, Receives from A and O
69
Type B
Antibody A, Antigen B, Receives from B and O
70
Type AB
Antibody none, Antigen AB, Receives from A,B,AB,O
71
Type O
Antibody A and B, Antigen: none, Receives from O
72
IgG
Passes through placenta
73
IgM
Cannot pass through placenta
74
Rh Factor
Rh present = positive (Antigen Rh +, Antibody Rh -) , Rh not present = negative ( Antigen Rh - Antibody Rh +)
75
No clump
negative
76
Clump
Positive
77
ELISA
Enzyme Linked Immuno Sorbant Assay

Microbio Lab Practical (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions