Chem 5 Flashcards
(11 cards)
What types of specimens are required for creatinine clearance?
Both blood and urine specimens
These specimens are necessary to accurately measure creatinine clearance.
What are the two main analytical methods for measuring creatinine?
Enzymatic and Chemical
These methods are used to determine creatinine levels in body fluids.
What is the chemical method for measuring creatinine known as?
Jaffe
The Jaffe method involves creatinine reacting with picric acid.
What complex is formed in the Jaffe reaction?
An orange-red complex
This complex is directly proportional to the concentration of creatinine.
Where is creatinine synthesized in the body?
Kidney, liver, and pancreas
These organs contribute to the endogenous production of creatinine.
Why is the measurement of creatinine clinically important?
Due to its endogenous production and constant release into body fluids
It is used to assess renal function.
What does renal clearance indicate?
Glomerular filtration rate (GFR)
It is a key indicator of kidney function.
List the enzymatic methods for measuring creatinine.
- Creatininase
- Creatinase
- Creatinine Deaminase
These methods involve a multistep approach leading to a photometric end point.
What is a limitation of the Jaffe reaction?
It is not specific for creatinine
Other substances can also react, leading to potential inaccuracies.
What substances can picrate react with besides creatinine?
- Proteins
- Glucose
- Ascorbic acid
- Acetone
- Acetoacetate
- Pyruvate
These reactions can interfere with the measurement of creatinine.
What methods have been developed to improve the specificity of the Jaffe method?
End point and kinetic methods
These advancements aim to enhance measurement accuracy.
