CHEM PHYS SB

Question 1

carboxylation is when added

Answer 1

carboxyl group

Question 2

structure of hydrquinone

Answer 2

benze and 2 oh groups

Question 3

velocity of ion depend on, fastest ion are those with ___ small/large ratio

Answer 3

mass to charge ration, m/z

Question 4

if we double the frequency what happens to wavelength

Answer 4

wave is halfed

Question 5

proteolytic cleavage is what reaction

Answer 5

hydrolysis

Question 6

this is when use energy released from what happen in chamber to heat water surrounding the chamber

Answer 6

calorimetry

Question 7

when the laser/photon energy is just right for chemical bond, no excess heat is released from bond breakages in the celll, and no sound waves produces, what is read on energy meter

Answer 7

0

Question 8

enthalpy is what kind of function and it is known as

Answer 8

state function, and known as energy too

Question 9

formula for kinetic energy of ejected electrons is

Answer 9

KE=HF–work function

Question 10

amine and excess carboxylic acid yields

Answer 10

amide and carboxylic acid

Question 11

to sepearte carboxy acid by cahrge we can deprotonate it with a

Answer 11

strong base

Question 12

phospatides contain both

Answer 12

phosphorus and choline head (nitrogen part)

Question 13

short reaction times favour ___ and long reaction time favour____

Answer 13

kinetic control, thermodynamic control

Question 14

kinetic contorl has ___ activation energy and is associated more with

Answer 14

lower, irreversible reaction

Question 15

thermodynamic control has more ___ and has ___ energy state and associated with

Answer 15

stable, lower, reversible

Question 16

ex, mixing liposomes of different sizes results in forming new liposome which are intermediate size indicate more thermodynamically state.

Answer 16

thermodynamic control

Question 17

double bond indicate there are overlapping n ___ orbitals and this delocalizes electrons

Answer 17

pi

Question 18

this is used to stabilize some enzymes during the digestion of dna, and prevent adhesion of enzyme to reaction tubes, tipc,

Answer 18

BSA - bovine serum albumin

Question 19

formula for catalytic efficiency is

Answer 19

kcat/km

Question 20

kcat measures

Answer 20

the number of molecules of substrate turned over into product per unity time

Question 21

lysozyme kill gram positive bactera by cleaving N-acetylglucosamine oligosaccharides what bond is lceaved

Answer 21

glycoside - oligo

Question 22

when performing experiments to measure kcat of an enzyme the substrate con must be

Answer 22

saturating - vmax

Question 23

at ph 7 histidine is

Answer 23

neutral

Question 24

if measuring color in experiment then use this technique to determine rate of reaction

Answer 24

monitor absorbance at oppositve colour

Question 25

glutamate ____ deprot/prot water

Answer 25

deprot

Question 26

when cleaving lactose which is made up of ___ and ____, the ____ get protanated, and the other get ______ then deprot water will attack galactose and sugar labeleled. this will produce this _____ only, and _____ is the leaving group

Answer 26

glucose and galactose, water/glucose, deprotonated, produce galactose only, and glucose is LG

Question 27

what expression will give concentration of lactase in prep for enzymes - what are steps

Answer 27

first, look at stock, and dilutions values. for example, stock is 0.1 ml and buffer is 25ml so 1--> 250 ml (1/250), then, further diluted 1 ml substrate mixed 1 ml enzyme colution so hald (1/2), now 1/250 times 1/2 = 1/500, so enzyme con is E X 500

Question 28

when numbering rings, have N at the

Answer 28

lowest number possible, so for cytosine, start from NH bottom, and then number counterclovk

Question 29

in _______ mixture is separated as it passess throuhgt a medium

Answer 29

chromatography

Question 30

use of histine tagging and nickel column is form of

Answer 30

affinity chroma

Question 31

to break 4 disulfide bonds (which get reduced) need how many

Answer 31

4 moles of NADH (reducing agent)

Question 32

where would estrogen (steorid) and peptide (insluin) be in hexane/wate phase

Answer 32

estrogen in hexane, and peptide in aqueous

Question 33

if strucute is more compact how far will it go in gel electrophoresis

Answer 33

farther

Question 34

where is Tm on graph

Answer 34

midpoint (tm is defined as point where 50% double stranded dna is changed to ssDNA)

Question 35

when seeing the stabilizing effect on graph with Tm need to look and see

Answer 35

lowest unfolding, and see the concentration labeled to see how storng solution is to break it

Question 36

if it says glycine used with hydrogen bonds it must have used is

Answer 36

backbone instead of side chain

Question 37

formula for catalytic efficiency

Answer 37

kcat/km

Question 38

galactose is a ___epimer of ____ which is an ____

Answer 38

C4, glucose, which is a six carbon aldose

Question 39

with pyranose rings being _____and furanose rings being ____

Answer 39

six-membered and five

Question 40

if Km is mich lower because

Answer 40

vmax is lower which means less substrate is needed to reach it

Question 41

adenine is ___ H donor and ___ acceptor

Answer 41

1,1

Question 42

Thymine has ___ H donor and ___acceptor

Answer 42

1,1

Question 43

Guanine has __ H donor and __ acceptor

Answer 43

2, 1

Question 44

Cytosine has ___ h donor, and ___ acceptor

Answer 44

1, 2

Question 45

these factors affect the Tm of dna in experiment

Answer 45

ph of solution, ionic strength, and length of dna strands

Question 46

which a.a. side chain can form a bond that is similar to peptide bond

Answer 46

lysine - charged R group form isopeptide bonds

Question 47

Lys is basic and would give ___ pI and Glu is acidic do it would give ____ pI

Answer 47

high, low

Question 48

maltose is made up of ___ and ____, and is non/red/or not

Answer 48

glucoe and glucose, is reducing, and only one anomeric carbon is involved

Question 49

for purification yield, need to multipole protin (mg) by units (units/mg) and

Answer 49

compare before and after (x/y)

Question 50

glutamate itself is acidic a.a so lowering ph to depor will/not affect it

Answer 50

not like wont change to activity

Question 51

going from ph 4 to 6 and state the hydrogn concentration from high ph ot low ph

Answer 51

0.01, theres lowe hydrogen con at ph of 6

Question 52

structure of glycerol 3-phosphate

Answer 52

hydroxyl on first 2 carbons and 3-phosphate

Question 53

higher kcat means

Answer 53

lower y int

Question 54

an ordered mechanism is when

Answer 54

specifc order to bidning, this glycerol bind first

Question 55

ternary means

Answer 55

3 parts formed tgt

Question 56

which peptide most likley to form a covalently bond dimer

Answer 56

cycteine

Question 57

more RBC interactions with wall yield ___ low/greater viscoity

Answer 57

greater

Question 58

small vessel diamter means ___ viscoity

Answer 58

lower

Question 59

more interactions and greater diamerter causes __ veclity

Answer 59

greater