Chemical Tests for Proteins Flashcards by Alyssa Abigail Fuentes

Q

Complete Hydrolysis

What amino acids are partially destructed in acid hydrolysis

A

cys
tyr

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Q

Complete Hydrolysis

What amino acid are completely destructed in acid hydrolysis

A

trp

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Q

Complete Hydrolysis

What amino acids are incompletely liberated in acids hydrolysis

A

val
ile

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Q

Complete Hydrolysis

What amino acids are destructed and racemized in acid hydrolysis

A

ser
thr

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Q

Complete Hydrolysis

What amino acids are converted to asp and glu in acid hydrolysis

A

asn
gln

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Q

Complete Hydrolysis

What amino acid is preserved or not destroyed in basic hydrolysis

A

trp

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Q

Complete Hydrolysis

What amino acids are completely destructed in basic hydrolysis

A

gln
asn
arg
ser

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Q

Qualitative Tests for Proteins

What test detects the presence of peptide bonds

A

Biuret test

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Q

Qualitative Tests for Proteins

What test has a positive result of pink to violet to blue coloration

A

Biuret test

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Q

Qualitative Tests for Proteins

What test has this principle: Complexation of Cu2+ with a peptide bond

A

Biuret test

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Q

Qualitative Tests for Proteins

What test detects the free α-amino acids and amines

A

Ninhydrin test

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Q

Qualitative Tests for Proteins

What test has the reagents: NaOH and CuSO4

A

Biuret test

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Q

Qualitative Tests for Proteins

What test has the reagents:
1,2,3-indanetrione monohydrate and EtOH

A

Ninhydrin test

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Q

Qualitative Tests for Proteins

What test has a positive result of:
- Blue or blue-purple color
- yellow-orange (Proline)

A

Ninhydrin test

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Q

Qualitative Tests for Proteins

What test has this principle: oxidative decarboxylation and deamination followed by condensation

A

Ninhydrin test

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Q

Qualitative Tests for Proteins

What test detects aromatic amino acids (Y, W, F)

A

Xanthoproteic test

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Q

Qualitative Tests for Proteins

What test has this principle: nitration of aromatic ring

A

Xanthoproteic test

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Q

Qualitative Tests for Proteins

What test has the reagents: conc. HNO3 & conc. NaOH

A

Xanthoproteic test

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Q

Qualitative Tests for Proteins

What test has a positive result of:
- yellow solution (heating)
- orange sol’n (excess NaOH)

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A

Xanthoproteic test

Q

Qualitative Tests for Proteins

What test detects phenolic ring (Y)

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A

Millon’s test

Q

Qualitative Tests for Proteins

What test has a positive result of: old rose/flesh, purple-red ppt

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A

Millon’s test

Q

Qualitative Tests for Proteins

What test has the reagents: HgSO4 in H2SO4

Hg (Mercury)

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A

Millon’s test

Q

Qualitative Tests for Proteins

What test has this principle: complexation (mercuration & nitration or nitrosation) and complexation of nitrohydroxylphenyl derivatives qith Hg2+

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A

Millon’s test

Q

Qualitative Tests for Proteins

What test has this principle: reduction of oxalate to glycolate and acid-catalyzed condensation of 2 trp’s with glyoxylic acid

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A

Hopkins-Cole test

# Qualitative Tests for Proteins What test has the reagents: **Glyoxylic acid and conc. H2SO4**

Hopkins-Cole test

# Qualitative Tests for Proteins What test has a positive result of: **pink to violet interface / ring in test tube**

Hopkins-Cole test

# Qualitative Tests for Proteins What test detects **indole group in Trypthophan (trp)**

Hopkins-Cole test

# Qualitative Tests for Proteins What test detects **guanidino/guanidine group of Arginine (arg)**

Sakaguchi test

# Qualitative Tests for Proteins What test has a positive result of: **Red to red-orange color**

Sakaguchi test

# Qualitative Tests for Proteins What test has the reagents: **α-napthol, NaOBr, NaOH, Urea**

Sakaguchi test

# Qualitative Tests for Proteins What test has this principle: **base-catalyzed of α-napthol (OA) with guanidino group**

Sakaguchi test

# Qualitative Tests for Proteins What test has this principle: **degradation and substitution reaction to form PbS**

Fohl's/Lead Acetate test

# Qualitative Tests for Proteins What test has the reagents: **Lead(II) acetate and NaOH**

Fohl's/Lead Acetate test

# Qualitative Tests for Proteins What test has a positive result of: **Brown or black ppt**

Fohl's/Lead Acetate test

# Qualitative Tests for Proteins What test detects **S-containing amino acids (M & C)**

Fohl's/Lead Acetate test

# Qualitative Tests for Proteins What test detects **presence of His and Tyr**

Pauly's test/Diazo reaction

# Qualitative Tests for Proteins What test has a positive result of: **Red color**

Pauly's test/Diazo reaction

# Qualitative Tests for Proteins What test has the reagents: **Sulfanic acid, NaNO2, Na2CO2**

Pauly's test/Diazo reaction

# Qualitative Tests for Proteins What test has this principle: **Diazotized sulfanilic acid coupled with amino phenol (forms a color red azo compound in cold)**

Pauly's test/Diazo reaction

# Qualitative Tests for Proteins What test has this principle: **Complexation**

Nitroprusside Test

# Qualitative Tests for Proteins What test has the reagents: **Na2Fe(CN)5NO in dilute NH3**

Nitroprusside Test

# Qualitative Tests for Proteins What test detects **presence of -SH group (C)**

Nitroprusside Test

# Qualitative Tests for Proteins What test has a positive result of: **red color**

Nitroprusside Test

# Qualitative Tests for Proteins What test has a positive result of: **red litmus paper to blue (basic)**

Amide test

# Qualitative Tests for Proteins What test detects **1°,2°,3° amides and nitriles**

Amide test

# Qualitative Tests for Proteins What test has the reagent/s: **NaOH**

Amide test

# Qualitative Tests for Proteins What test has this principle: **Base-catalyzed hydrolysis**

Amide test