chloroplasts Flashcards
1
Q
state the 4 layers of the chloroplast from outer to innermost
A
- outer membrane
- inter membrane space
- inner membrane
- stroma
2
Q
what are granum?
A
stacked thylakoids
3
Q
what are thylakoids?
A
folded membranes containing photosynthetic pigments involved in light dependent reactions
4
Q
what is the stroma?
A
fluid interior containing enzymes involved in the light independent reaction
5
Q
what are the primary pigments?
A
chlorophyll a and b
6
Q
what are the accessory pigments?
A
carotenoids
7
Q
chlorophyll a absorbs which colours?
A
yellow
green
8
Q
chlorophyll b absorbs which colours?
A
blue
green
9
Q
caratenoids absorb which colour?
A
orange
10
Q
where are pigments located?
A
embedded in thylakoid membrane
11
Q
what do the pigments do?
A
forms light harvesting systems and each pigment absorbs different wavelengths of light
12
Q
summarise the process of separating pigments using TLC
A
- crush leaf and grind with sand & propanone to extract pigments
- transfer to watch glass & dry to evaporate moisture
- add propanone to absorb pigments
- add spot to TLC paper and place tip in running solvent
