chromatograohy

Question 1

Covers physical-chemical methods of separation and analysis of mixtures of gases, vapor, liquids or dissolved substances

Answer 1

Chromatography

Question 2

They work because of the

Answer 2

The differences in distribution of mixture components between the mobile phase and stationary phase, when the mixture is moved through the stationary phase, the layer of the sorbent.

Question 3

Components of the analyzed mixture are not _____on the sorbent; compounds with the higher affinity to the sorbent will be adsorbed more _________ on the sorbent, therefore the speed of their moving with the mobile phase is ____.

Answer 3

equally adsorbed
strongly and stay for longer
slower

Question 4

Stationary phase is the______

it can be ____ or ____

Answer 4

can be liquid or solid.

Sorbent

Question 5

Mobile phase is the___

performs the role of _______ and

Answer 5

(the gas or liquid passing the layer of the sorbent)

solvent and carrier of analysis mixture

Question 6

Methods of Chomatography

Answer 6

.1. According to the physical nature of a mobile phase:
• liquid chromatography (when a mobile phase is liquid);
• gas chromatography (when a mobile phase is a gas).

2. According to the mechanism of interaction between the material and the sorbent:
3. According to the way of chromatographing
4. According to the applied techniques:

Question 7

According to the mechanism of interaction between the material and the sorbent includes?

Answer 7

adsorption chromatography (divided in turn into molecular chromatography where the interaction is based on intermolecular forces of Van-der-Waals;

Chemosorption chromatography, where the adsorption is caused by various chemical reactions: ion exchange, precipitation, complexation, redox, etc.);

Gel-chromatography (separation of mixture components due to diffusion of molecules of dissolved materials into the pores of the sorbent).

Question 8

Separation of mixture components due to diffusion of molecules of dissolved materials into the pores of the sorbent

Answer 8

Gel-chromatography

Question 9

Divided in turn into molecular chromatography where the interaction is based on intermolecular forces of Van-der-Waals

Answer 9

Adsorption chromatography

Question 10

Where the adsorption is caused by various chemical reactions: ion exchange, precipitation, complexation, redox, etc.);

Answer 10

Chemosorption chromatography

Question 11

According to the way of chromatographing includes

Answer 11

frontal chromatography;
elution chromatography;
displacement chromatography, etc.

Question 12

4. According to the applied techniques:

.

Answer 12

planar chromatography (thin-layer chromatography, chromatography in paper);
column chromatography;
capillary chromatography, etc

Question 13

Nowadays, for the analysis of proteins and other materials filtration through _______is widely used. In this case a stationary phase is a _____ in pores (small cavities) of a solid sorbent.

Answer 13

gels (gel chromatography)

liquid

Question 14

The size of pores determines the ________ able to get into them, thus the length of the way for the material to pass through the column depends on its ______

Answer 14

volume of molecules

molecular weight

Question 15

is based on the different solubility of components of analysis mixture in non-mixing liquids: mixtures of water and organic solvents.

Answer 15

Chromatography in paper

Question 16

The ratio between the length of the component way and that of the solvent is called_____ and in normal conditions is a____.

Answer 16

distribution coefficient (Rf) and in normal conditions is a constant.