Chromatography Flashcards
What is chromatography?
A technique used to separate mixtures into their individual components.
True or False: Chromatography can be used for both qualitative and quantitative analysis.
True
Fill in the blank: In chromatography, the stationary phase is typically a _______.
solid or a liquid coated on a solid
What are the two main phases in chromatography?
Stationary phase and mobile phase
Multiple Choice: Which of the following is a type of chromatography? A) Gas chromatography B) Liquid chromatography C) Both A and B
C) Both A and B
What is the purpose of the mobile phase in chromatography?
To carry the analyte through the stationary phase.
True or False: In paper chromatography, the stationary phase is paper.
True
What is Rf value in chromatography?
The ratio of the distance traveled by the compound to the distance traveled by the solvent front.
Fill in the blank: The process of separating components by their different affinities for the stationary and mobile phases is called _______.
partitioning
Multiple Choice: Which factor does NOT affect the separation of components in chromatography? A) Temperature B) Solvent polarity C) The color of the components
C) The color of the components
What is the main application of chromatography in the field of biochemistry?
To separate and analyze biomolecules such as proteins and nucleic acids.
True or False: Column chromatography uses a vertical column filled with stationary phase.
True
What is the primary use of thin-layer chromatography (TLC)?
To quickly analyze small samples and monitor the progress of reactions.
Fill in the blank: The technique that uses gas as the mobile phase is called _______.
gas chromatography
What does the term ‘elution’ refer to in chromatography?
The process of washing out the components from the stationary phase using the mobile phase.
