Chromatography Flashcards
What is chromatography used for?
Chromatography can be used to separate and identify the components in a mixture.
What is thin layer chromatography?
Can be used to separate and identify the components in a mixture.
What is column chromatography?
A column is packed with a solid and a solvent moves down the column
What is gas chromatography?
A column is packed with a solid or with a solid coated by a liquid, and a gas is passed through the column under pressure at high temperature
What does separation depend on?
The balance between solubility in the moving phase and retention by the stationary phase. (Attraction of a molecule to the stationary phase)
What is used to identify different substances?
Rf values and retention times
What organic analysis method is used to identify components separated by GC?
Mass spectrometry
