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Shifting Exams (MACHINES) > CLINICAL CHEMISTRY > Flashcards

CLINICAL CHEMISTRY Flashcards

1
Q

Serum Samples and their tube Color

A

Routine Chem Tests (R/Y)
Random Blood Sugar
2 Post Prandial Blood Sugar
Oral Glucose Tolerance Test
Therapeutic Drug Monitoring (R)
Blood Alcohol (R)
Homocysteine (R)

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2
Q

Therapeutic Drug Monitoring

A

Cortisol
Vancomycin
Gentamicin
Digoxin
Valproic Acid
Methotrexate
Carbamazepine
Phenobarbital
Phenytoin

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3
Q

Plasma Samples (requires centrifugation)

A

lactate assay (gray)
Plasma ammonia (green)
NT-Pro BNP (green)
Troponin I (green - Lithium Heparin)

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4
Q

5 Tests that requires WHOLE BLOOD sample

A

HbA1c
Tacrolimus
Sirolimus
Cyclosporine
Troponin T

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5
Q

Tests that do not require centrifugation

A

WHOLE BLOOD samples such as:
Troponin T
HBA1C
CYCLOSPORINE
TACROLIMUS
SIROLIMUS

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6
Q

How many drops of HCl must be added to urine samples

A

2-3 drops and then invert tube 2-3 times also

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7
Q

Purpose of adding HCl in urine

A

Acidify and preserve samples

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8
Q

24 hr metabolic urine includes

A

Metanephrine
VMA
5-HIAA
Catecholamine

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9
Q

What specimen requires noting of bottle number

A

CSF
Ascitic/Peritoneal
Pleural
Pericardial

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10
Q

Routine 24 hr urine tests

A

Oxalate
Amylase
Calcium
Chloride
Magnesium
Phosphorus
Potassium
Sodium
Urea Nitrogen
Uric Acid
Glucose
Protein
Protein-Creatinine Ratio
Creatinine
Endogenous creatinine clearance

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11
Q

Send out to SLMC Global

A

24 hr metabolic urine: metanephrine, HIAA, VMA, Catecholamine
Glycomark (1,5 anhydroglucitol - R/Y)
Amikacin - Red top

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12
Q

Tests requiring RED TOP only

A

Therapeutic Drug Monitoring
Blood Alcohol
Homocysteine
Amikacin

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13
Q

HBA1C test (machine and principle)

A

ARKRAY - HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

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14
Q

Sample and reagents for HBA1C

A

5 ul WHOLE blood
450 ul diluent 80

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15
Q

Serum and Random Urine Osmolality (machine and principle)

A

3320 Osmometer - Freezing Point Osmolality

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16
Q

Ionized Calcium (machine and principle)

A

NOVAPRIME - Ion Selective Electrode (ISE)

17
Q

ATELLICA SCI principle in chemistry

A

Spectrophotometry

18
Q

TDM, Tacrolimus, Sirolimus and Cyclosporine is done in what machine

A

ARCHITECT - Chemiluminiscence Microparticle Assay

19
Q

Principle of Diazyme DZ Lite

A

Chemiluminescence Immunoassay

20
Q

Adenosine Deaminase uses what machine

A

Diazyme DZ Lite

21
Q

Specimen for Adenosine Deaminase (ADA)

A

Pleural, Ascitic, Pericardial, CSF

22
Q

Centrifuge (rpm and mins)

A

4000 rpm
15 mins

23
Q

Atellica DLC principle

A

Spectrophotometry

24
Q

Tacrolimus measurement of sample and reagent

A

Tacro = 200 ul WB + 200 ul precip

25
Q

Cyclosporine measurement of sample and reagent

A

Cyclo = 200 ul WB + 100 ul solubilization rgnt + 400 ul precipitation rgnt

26
Q

Sirolimus measurement of sample and reagent

A

Siro = 150 ul WB + 300 ul precip rgnt

27
Q

Microcentrifuge of tacrolimus

A

14000 rpm
4 mins

28
Q

Microcentrifugation of cyclosporine and sirolimus

A

10,000 rpm
4 mins

Shifting Exams (MACHINES) (5 decks)

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