clinical testing Flashcards
(10 cards)
what patient details do you need for sampling
Name
DOB/Age
NHS Number
Sex
Contact with infectious disease
Foreign Travel
Antibiotic therapy
Symptoms
Tests required
what to do to get a quality sample
When it was taken
How much was taken
Presence of other microorganisms
Aseptic techniques
Correct preparation of the body site
Storage container
The correct specimen
what are the transport conditions for samples
Time taken to get the specimen to the lab
Temperature
Storage
Maintenance media
Charcoal swabs/viral transport
Maintains a specimen in a similar state until it is processed
what physical conditions are essential for culturing
- time, temp, gas, moisture, light.
what are the other considerations for culturing
cross reactions, specimens, atypical clinical presentation, some pathogens have never been grown in culture, others only grow intracellularly in tissue culture.
what lab tests can be done
Culture
Microscopy
Detection of bi-products
Molecular techniques
what is selective in cultures
- media and physical conditions
- enrichment
- incubation
what is vibrio cholerae cultured in
alkaline peptone water
what is salmonella cultured in
selenite broth
