COLONIAL MORPHOLOGY

Question 1

QUALI OR QUANTI

what type of isolation is used for urine culture?

Answer 1

quantitative

because you do COLONY COUNTING

Question 2

T/F:

Hemolysis can be seen on both Blood Agar and Choco Agar plates

Answer 2

False

BAP only. Choco agar RBC are already lysed while heating

Question 3

Enumerate:

3 types of hemolysis in BAP

Answer 3

• alpha
• beta
• gamma

Question 4

hemolysis

partial; greenish zone

Answer 4

alpha hemolysis

example: S. pneumoniae

Question 5

hemolysis

complete; clear zone

Answer 5

beta hemolysis

examples: S. pyogenes, S. aureus

Question 6

hemolysis

no hemolysis

Answer 6

gamma hemolysis

example: E. faecalis

Question 7

Score of a plate with colonies visible only in quadrant 1, with fewer than 10 colonies?

Answer 7

1+

Question 8

score of a plate with colonies visible in quadrants 1 and 2, with more than 10 colonies in quadrant 1 and fewer than 10 in quadrant 2?

Answer 8

2+

Question 9

If colonies are present in quadrants 1, 2, and 3, and more than 10 colonies are observed in quadrants 1 and 2 but fewer than 10 in quadrant 3, what is the score?

Answer 9

3+

Question 10

A plate shows colony growth in all four quadrants, with more than 10 colonies in each quadrant, what is the score?

Answer 10

4+

Question 11

Give an example of Double Zone of Hemolysis

Answer 11

C. perfringens

Question 12

enumerate

sizes of colonical morphology (3)

Answer 12

• pinhead
• pinpoint
• large, medium

Question 13

sizes

typical of staphylococci

Answer 13

pinhead

Question 14

sizes

typical of streptococci

Answer 14

pinpoint

Question 15

sizes

tend to be G(-) bacilli

Answer 15

large, medium

Question 16

enumerate

colony shapes

Answer 16

• punctiform (pinpoint)
• circular
• filamentous
• irregular
• rhizoid
• curled

Question 17

enumerate

elevation

Answer 17

• flat
• raised
• convex (dome)
• umbilicate
• umbonate

Question 18

enumerate

margins

Answer 18

• entire
• undulate
• filamentous
• lobate
• erose
• curled
• scalloped

Question 19

dry, wrinkled, breadcrumb-like

Answer 19

Nocardia spp.

Question 20

hammered copper

Answer 20

Yersinia pestis

aka plague bacilli

Question 21

identify

what bacteria shows a green, pearlescent appearance on a BAP?

Answer 21

P. aeruginosa

Question 22

Enumerate

pigments produced by Pseudomonas (4)

Answer 22

• pyoverdine
• pyocyanin
• pyorubin
• pyomelanin

Question 23

color

pyoverdine

Answer 23

green

though **yellow-green **yung sabi ni pubmed

Question 24

color

pyocyanin

Answer 24

blue

Question 25

# color pyorubin

Answer 25

red | red-brown sabi ni pubmed

Question 26

# color pyomelanin

Answer 26

black/brown

Question 27

Pedicle/footlike

Answer 27

*Candida albicans* ## Footnote this is not a bacteria, it's YEAST

Question 28

Density of a colony can be: (3)

Answer 28

* transparent * translucent * opaque ## Footnote to see diff densities, look through the colony while using **transillumination**

Question 29

This bacteria produces semi-opaque colonies, with organisms concentrated at the center | sometimes called "bull's eye"

Answer 29

*Streptococcus agalactiae*

Question 30

# T/F: Staphylococci are usually translucent

Answer 30

False | opaque ## Footnote source: mahon

Question 31

This bacteria is described as shiny (like a half-pearl) on blood-containing media

Answer 31

*Bordetella pertussis* ## Footnote source: mahon

Question 32

# color or pigment this is used to describe a **particular genus** in general

Answer 32

color

Question 33

# color or pigment an inherent characteristic of a specific organism confined generally to the colony, but will sometimes diffuse through the culture medium

Answer 33

pigment

Question 33

# color coagulase negative staphylococci

Answer 33

white

Question 34

# color G(-) rods, *Enterococcus spp.*

Answer 34

gray

Question 35

# color *S. aureus, Micrococcus spp*., non-pathogenic *Neisseria spp.*

Answer 35

yellow or off-white

Question 36

# color diphtheroids

Answer 36

buff

Question 37

thioglycolate broth is stored at what temp?

Answer 37

room temp

Question 38

# liquid media if the growth is all throughout the broth..

Answer 38

facultative

Question 39

# liquid media if the growth is at the top only

Answer 39

obligate aerobe

Question 40

# ODOR *Proteus*

Answer 40

burnt chocolate/chocolate cake

Question 41

# ODOR *S. aureus*

Answer 41

old sock

Question 42

# ODOR bleach

Answer 42

*E. corrodens* | eikenella

Question 43

# ODOR corn taco/grapefruit

Answer 43

*Pseudomonas aeruginosa*

Question 44

# ODOR *P. multocida*

Answer 44

musty

Question 45

# ODOR almond like

Answer 45

*Chromobacterium violaceum*

Question 46

# ODOR barnyard

Answer 46

*C. difficile*

Question 47

# ODOR popcorn like

Answer 47

*Neisseria animaloris*

Question 48

# ODOR *Nocardia*

Answer 48

freshly plowed field

Question 49

# ODOR Haemophilus

Answer 49

musty basement

Question 50

# ODOR *A. faecalis*

Answer 50

freshly cut apple/strawberry

Question 51

# FORM/MARGIN medusa head

Answer 51

*Bacillus anthracis*

Question 52

# FORM/MARGIN *Eikenella corrodens*

Answer 52

fuzzy edged

Question 53

# FORM/MARGIN Proteus spp.

Answer 53

swarmers

Question 54

# FORM/MARGIN wagon-wheel

Answer 54

*Moraxella catarrhalis*

Question 55

# FORM/MARGIN Actinomyces

Answer 55

molar-tooth appearance

Question 56

# FORM/MARGIN *M. tuberculosis*

Answer 56

cauliflower-like

Question 57

# FORM/MARGIN *C. albicans*

Answer 57

foot/pedicle

Question 58

# FORM/MARGIN *Yersinia pestis*

Answer 58

stalactite pattern

Question 59

# CONSISTENCY Nocardia spp.

Answer 59

brittle (forms splinters; breadcrumb like)

Question 60

# CONSISTENCY S. aureus

Answer 60

creamy/buterous

Question 61

# CONSISTENCY Diphtheroids

Answer 61

dry and waxy

Question 62

# CONSISTENCY sticky

Answer 62

*Neisseria spp.*

Question 63

# CONSISTENCY beaten egg white

Answer 63

*B. anthracis*

Question 64

# CONSISTENCY mucoid

Answer 64

capsulated organisms *(Klebsiella pneumoniae)*

Question 65

Serratia marcesens is red due to..

Answer 65

prodigiosin

Question 66

pigment of C. violaceum

Answer 66

purple

Question 67

convex, entire edge, 2-3mm, creamy, yellowish, beta-hemolytic

Answer 67

Staphylococcus ## Footnote basta may creamy, almost always staph:D

Question 68

convex/pulvinate, translucent, pinpoint, butyrous, wide zone of beta-hemolysis

Answer 68

Streptococcus

Question 69

umbilicate or flat, translucent, butyrous or mucoid, broad zone of alpha hemolysis

Answer 69

Pneumococcus

Question 70

pulvinate, semiopaque, gray, moist to dry, beta-hemolysis may or may not be present

Answer 70

E. coli & other enterics

Question 71

flat, gray, spreading as a thin film on agar, **burn chocolate **odor

Answer 71

Proteus

Question 72

flat, gray to **greenish**, margins erose or spreading, green-blue pigment, **grapelike** odor

Answer 72

Pseudomonas