Common Laboratory Equipment Flashcards
(95 cards)
1
Q
3 Pipette Classification
A
- According to Calibration
- According to Dispensing
- According to Use
2
Q
2 types of pipette according to Calibration?
A
- To Contain
- To Deliver
3
Q
It holds particular volume but does not dispense the exact volume
A
TC Pipette
4
Q
TC pipette is calibbrated by?
A
Introducing exact weight of mercury at a specific temperature
5
Q
This pipette dispenses the indicated volume
A
TD Pipette
6
Q
TD Pipette is calibrated by?
A
Weighing the volume of water that flows by gravity
7
Q
T/F: TD pipette rate of delivery must be hastened by blowing.
A
False (Must not be hastened)
8
Q
2 Types of pipette based on Dispensing
A
- To Blow-Out
- Self- Draining
9
Q
A pipette with an etched ring near the mouthpiece
A
Blow Out Pipette
10
Q
The user allows the contents of the pipette to drain by gravity
A
Self Draining Pipette
11
Q
6 Types of pipette according to use
A
- Volumetric Pipette
- Ostwald-Folin
- Graduated Pipette
- Micropipette
- Pasteur Pipette
- Automatic Pipette
12
Q
Has the greatest degree of accuracy and precision
A
Volumetric Pipette
13
Q
Volumetric Pipette is also known as?
A
Transfer pipette
14
Q
Transfer Pipette is calibrated to deliver a ______ volume of liquid
A
Fixed
15
Q
This has a bulb between mouthpiece and tip that decreases surface area/unit volume.
A
Transfer Pipette
16
Q
T/F: Volumetric Pipette is a self draining pipette.
A
True
17
Q
It is used to measure viscous fluid and has a bulb near the tip
A
Ostwald-Folin Pipette
18
Q
What volume does Ostwald-Folin Pipette measure?
A
2.0mm or Less
19
Q
2 Physical Characteristics of Ostwald-Folin Pipette
A
- Bulb near Tip
- Etch Mark, ring near mouthpiece
20
Q
T/F: Ostwald-Folin is a Blow-Out pipette
A
True
21
Q
It is used to deliver an amount of liquid contained between two calibration on marks
A
Graduated Pipette
22
Q
Graduated Pipette is also known as?
A
Measuring Pipette
23
Q
2 Types of Graduated Pipette
A
- Serologic
- Mohr
24
Q
This pipette has an etched band on the suction piece
A
Serologic Pipette
25
This Graduated Pipette is a self draining pipette
Mohr
26
This Graduated pipette has calibration marks to the tip
Serologic
27
This Graduated Pipette is a blow-out pipette
Serologic
28
This Graduated Pipette has no graduations to the tip and the calibration lies between two marks in the stem
Mohr
29
It is used when small amount of specimen is needed (<1ml)
Micropipette
30
Pipette with no calibration and used for specimens without specific volume
Pasteur Pipette
31
Most Routinely used pipette
Automatic Pipette
32
6 Advantages of Automatic Pipette
1. Stability
2. Safety
3. Time Saving
4. Easy to use
5. Increase Precision
6. Lack of Required Cleaning
33
3 Types of Automatic Pipette
1. Air-Displacement
2. Positive Displacement
3. Dispenser
34
Automatic Pipette that relies on a piston for creating suction
Air-Displacement Pipette
35
Operates by moving the piston in the pipette tip or barrel
Positive Displacement Pipette
36
This obtains liquid from a common reservoir
Dispenser/Dilutor
37
Designed for use with Air-Displacement Pipette
Pipette tips
38
Positive displacement pipette used tips made up of?
Straight Columns of Glass or Plastics
39
3 Quality Control Methods
1. Gravimetric & Spectrometric Method
2. 0.1% Phenol Red Solution in DH²O
3. Acid Dichromate and Nitric Acid
40
Used to Compare the reproducibility of brands of Pipette tips
0.1% Phenol Red Solution
41
Used to verify pipette volume accuracy and precision
Gravimetric and Spectrometric Method
42
Cleaning solution for glasswares
Acid Dichromate and Nitric Acid
43
Beakers should be made of glass that is ______ and ____
1. Resistant to many chemicals
2. Resistant to Heat
44
Used for general mixing and reagent prep
Beaker
45
Used to measure volumes of liquids when high degree of accuracy is not essential
Graduated Cylinder
46
Pear Shaped flasks that is used to contain a specific amount of liquid
Volumetric Flasks
47
2 Equipments Used for Measuring Mass
1. Analytical Balance
2. Rough or Platform Balance
48
The precision of Analytical Balance
1/1000 of a gram
49
Precision of Platform Balance
0.1 Gram
50
2 Examples of Platform Balance
1. Torsion Balance
2. Triple Beam Balance
51
It is the process of using centrifugal force to separate the lighter portions of a solution, mixture, or suspension from heavier portions
Centrifugation
52
6 Uses of Centrifuge
1. Remove Cellular elements from blood
2. Concentrate Cellular Elements for Microscopy
3. Remove Precipitated protein
4. Separate CHON bound / Ab-bound ligand
5. Extracts solutes from aqueous to organic solvents
6. Separate Lipid Components
53
6 Uses of Centrifuge
1. Remove Cellular elements from blood
2. Concentrate Cellular Elements for Microscopy
3. Remove Precipitated protein
4. Separate CHON bound / Ab-bound ligand
5. Extracts solutes from aqueous to organic solvents
6. Separate Lipid Components
54
3 Types of Centrifuge
1. Fixed Angle Head
2. Swinging Bucket Type
3. Ultracentrifuge
55
Other term for Fixed-Angle Head Centrifuge
Angle-Head
56
Other term for Swinging-Bucket Type Centrifuge
Horizontal Head
57
The force required to separate two phases
Relative Centrifugal Force (RCF)
58
RCF is equal to?
1.118 x 10^-5 x r x rpm²
59
3 Factors that affect RCF
1. Rotor Speed
2. Radius of the Rotor
3. Effective Path Length (Depth of Liquid)
60
Speed of centrifuge should be checked at least _______
Once every 3 Months
61
2 Equipment used to check the speed of the centrifuge
1. Tachometer
2. Strobe Light
62
Centrifuge timer should be checked ____ against a ______
1. Weekly
2. Reference Timer
63
Centrifuge Timer should be checked ______
Monthly
64
2 Types of Liquid-in-glass thermometer
1. Total Immersion
2. Partial Immersion
65
Type of Thermometer that monitors freezers and refrigerator
Total Immersion
66
What does Partial Immersion thermometer monitor? (2)
1. Heating Blocks
2. Water Bath
67
Used in freezing point depression osmometers
Thermistors
68
Temperature monitoring devices should be verified for accuracy at _______ or _____ intervals
1. 6 Months
2. 12 Months
69
TC pipette is also known as?
Rinse Out Pipette
70
3 Examples of TC pipette
1. Sahli
2. Lang-Levy
3. WBC/RBC Pipette
71
Graduated or Measuring Pipette is also known as?
Between 2 Marks Pipette
72
2 Types of Volume and their value for Automatic Pipette
1. Macro = >1ml
2. Micro = <1ml
73
2 Other types of Automatic Pipette
1. Single Channel
2. Multi Channel
74
Pipette tip color for <10ul?
White
75
What color of pipette tip is for 1000ul?
Blue
76
Yellow pipette tip messures up to what ul?
100ul
77
What does Gravimetric Quality Control Method use for QC? (2)
1. Analytical Balance
2. Class II Weighs
78
What does Spectrometric Method use for QC?
Potassium Dichromate Solution
79
What liquid is used for rinsing Glasswares?
Distilled Water
80
Glass used for beakers
Borosilicate Glass
81
At what angle does Fixed-Angle Head Centrifuge work?
25-52 Degrees
82
Where does the sediment reside in the tube when using fixed angle head centrifuge?
Bottom Side of Tube
83
Disadvantage of Fixed-Angle Head Centrifuge?
Latent Disturbance of Sediments
84
Where does the sediment inside the tube reside using Swinging bucket type Centrifuge?
Bottom of the tube
85
2 Provisions of Ultracentrifuge?
1. Higher RPM
2. Temperature Control
86
Temperature for 5k-10k rpm Centrifuge?
5°C
87
Rotor Speed: the _____ the speed, the ____ time needed
The Lower the Speed the Higher the Time needed
88
2 Types of Thermometers
1. Liquid-in-glass Thermometer
2. Thermistors
89
NIST meaning
National Institute of Standards and Technology
90
2 Types of Thermometer Calibrators
1. NIST SRM 934
2. NIST SRM 1968
91
SRM 934 is based on?
Scale 1990 (ITS-90)
92
Scale 1990 ranges at what temps? (4)
1. 0°c
2. 25°c
3. 30°c
4. 37°c
93
NIST SRM 1968 is also known as?
Gallium Melting point cell
94
How many grams of pure gallium does SRM 1968 have?
25g
95
Fixed melting point of SRM 1968
29.7646 °c
