Complete Blood Count [CBC]

Question 1

Congenital heart disease will increase or decrease RBC count

Answer 1

Increase

Question 2

The most common diluent in RBC counting

Answer 2

Isotonic saline

Question 3

The most preferred diluent in RBC counting

Answer 3

Davies

Question 4

An apparatus used in the gasometric method.

This is used to determine what

Answer 4

Natelson microgasometer

Question 5

The most reliable and recommended method to determine hemoglobin

Answer 5

Cyanmethemoglobin

Question 6

Another name for the cyanmethemoglobin method

Answer 6

Hemiglobincyanide

Question 7

Myeloma protein, abnormal globulins, and radiographic media may affect the accuracy of what hemoglobin determination method

Answer 7

Copper sulfate method (specific gravity)

Question 8

What type of hemoglobin is not measured in the hemiglobincyanide method ?

Answer 8

Sulfhemoglobin

Question 9

Blood sample and anticoagulant used in the cyanmethemoglobin method

Answer 9

Whole blood, EDTA

Question 10

What increases the alkalinity when abnormal globulins are present in px sample

Answer 10

Potassium carbonate

Question 12

The absorbance of the solution in the hemiglobincyanide method is measured in how many nanometers using the spectrophotometer

Answer 12

540 nm

Question 13

How do you resolve presence of hemoglobin C and S during CBC

Answer 13

Dilute with equal parts water and multiply the result by 2

Question 15

Reagent for the cyanmethemoglobin method

Answer 15

Modified drabkins

Question 16

Reagent for the sahli’s acid hematin method

Answer 16

Drabkin’s solution

Question 17

True or False

1. Hematocrit reflects the concentration of RBC
2. Hematocrit reflects the total red cell mass

Answer 17

1. T

2. F

Question 18

The reference indicator for calibration blood counting systems

Answer 18

Hematocrit

Question 19

Increased or Decreased hematocrit

1. Dehydration

Answer 19

Increase - relative concentration due to less plasma

Question 20

An over-anticoagulated sample results in an ________ (high or low)hematocrit

Answer 20

Low

Question 21

To obtain the packed cell volume: we set the centrifuge at _____ rpm for ____ minutes

Answer 21

10,000 to 15,000 rpm

5 min

Question 22

Improper flushing of an IV catheter will result to a false _____ (increase or decrease) in hematocrit value

Answer 22

False decrease

Question 23

How many ml is the capacity of a capillary tube

Answer 23

0.5 mL

Question 24

How much (in cm) should you fill a capillary tube when measuring Hct

Answer 24

5 cm

Question 25

Reference range for MCH

Answer 25

24-34 pg

Question 26

Sample used in platelet count

Answer 26

Whole blood EDTA

Question 27

1% ammonium oxalate reagent used in PLT count is use to ______

Answer 27

Lyse RBC

Question 28

Reese-Ecker method for PLT counting uses: 1. Reagent ______ 2. Stain _______ 3. Microscope_____

Answer 28

1. Citrate formaldehyde (buffer) 2. Brilliant cresyl blue 3. Phase contrast

Question 29

Guy and leaks method in PLT count uses: 1. Reagent _____ 2. Stain _____ 3. Microscope ______

Answer 29

1. Oxalate formaldehyde 2. Crystal violet 3. Light microscope

Question 30

In PLT estimating, how many OIO fields do we count ______ and how many RBCs _____

Answer 30

10 fields | 200 RBCs

Question 31

Hemolytic Uremic syndrome may cause a false ______ (increase or decrease) in PLTs

Answer 31

Decrease

Question 32

What chemical found in the modified drabkins reagent that converts methemoglobin to cyanmethemoglobin

Answer 32

Potassium cyanide

Question 33

What chemical in modified drabkin’s makes ferrous iron become ferric

Answer 33

Potassium ferricyanide

Question 34

The name of the chemical method in determining hemoglobin

Answer 34

Kennedy and Wong

Question 35

The hypochromia grading when the central pallor is 3/4 the cell

Answer 35

3+

Question 36

2+ hypochromia grading indicates ____central pallor

Answer 36

2/3

Question 37

True or False: | 1. Absorbance is INDIRECTLY proportional to the hemoglobin concentration

Answer 37

False

Question 38

How many percent of adult hemoglobin may be inactive. Hence may not be accurately measure in the gasometric method

Answer 38

2-12%

Question 39

Using a patient blank (px sample + hemiglobincyanide reagent) resolves what condition

Answer 39

Lipemia

Question 40

How many WBC and PLT in a sample needs to be centrifuged and re-read again (reading the supernatant)

Answer 40

>20 wbc | > 700 plt

Question 41

What reagent is used to speed up testing in the cyanmethemoglobin method

Answer 41

Dihydrogen potassium