Conformational Dynamics

Question 1

what is mass spectrometry

Answer 1

It is a process that weights molecules in space. A magnet will deviate moving electron beams from a sample from a path they are on and the degree that it deviates from the path will give an idea about the mass of the molecule.

Question 2

What is deuterium

Answer 2

a hydrogen isotope

Question 3

What is the hydrogen-deuterium exchange

Answer 3

Deuterium will exchange with hydrogen in water.

Question 4

True or False. Hydrogens in bonds will exchange quickly

Answer 4

False. They will either not exchange or do so very slowly

Question 5

True or False. Hydrogens outside of bond will exchange quickly

Answer 5

true

Question 6

What does it say about a protein if D is not incorporated quickly

Answer 6

The protein is less dynamic or highly involved in hydrogen binding

Question 7

What can the use of hydrogen-deuterium tell you about a protein

Answer 7

it tells you about the conformational flexibility. it can tell you about protected/conserved or highly interacting sites because exchange will not occur in these areas.