Connect Questions Flashcards
(51 cards)
ORF refers to…
The region of a gene that codes for a protein or a functional RNA product
Two or more genes that are so alike that they probably came from gene duplication are called….
Paralogs
Shotgun approach to determine sequencing is….
Done with powerful computers and automated sequencing
DNA microarray is used for….
Measure Levels of specific RNAs expressed by cells
The proteome refers to….
All of the proteins that an organism produces.
Isoelectric focusing…
Separates proteins based on pH
Protein spot eluted from gel analysis can be identified to specific genes that code for them using….
Mass spectrometry
Multiple strand displacement amplification is a powerful tool because…
It allows single-cell genomic sequencing that can analyze uncultivated microorganisms
Represents the entire genome visually
Physical genome map
Used to identify the DNA to which a protein binds
ChiP-Chip and ChIP-Seq analysis
Physical genome map color codes for…
Functional roles
Strand displacement is used to….
Copy the genome of a single microbial cell so there is enough DNA for sequencing
The microbiome….
Is the collection of microorganisms that are present in and on an organism
The determination of the taxon to which an organism belongs is called
Identification
Classification system based on mutual similarity characteristics
Phenetic
A classification system based on evolutionary relationships
Phylogenetic
Population descending from a single organism
Strain
Linnaeus
E. Coli
Binomial naming system of nomenclature assigns
Genus and species
Mole percent (G+C) determines relatedness at
Genus level
Stable annealing by hydrogen bonding between DNA from different organisms
Hybridization
Temp at half the strands separate
Melting temp
Analysis of DNA fragmentation patterns
Genetic fingerprinting
Classical characteristic fro taxonomy
Molecular characteristics
