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Histology Exam > Connective Tissue > Flashcards

Connective Tissue Flashcards

(61 cards)

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1
Q

What composes the extracellular matrix?

A
  • Fibers
  • Ground substance
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2
Q

What is the function of connective tissue?

A

Provide structural and metabolic support for the other tissues and organs.

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3
Q

What are the 5 different types of connective tissue?

A
  • Fibrous connective tissue
  • Adipose tissue
  • Cartilage
  • Bone
  • Blood
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4
Q

Which cells compose the connective tissue?

A
  • Mast cells
  • Fibroblast
  • Histocytes
  • Adipose cells
  • Reticular cells
  • Osteoblast/cytes
  • Chondroblast/cytes
  • Blood cells
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5
Q

What is the purpose of elastic fibre?

A

Allows stretching in tissue

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6
Q

What is the purpose of reticular fibres?

A

Delicate supporting framework for cellular organs

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7
Q

What is the purpose of collagen?

A
  • strength
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8
Q

What composes type 1 collagen?

A
  • Tendon
  • bone
  • adult skin
  • aorta
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9
Q

What composes type 2 collagen?

A
  • Cartilage
  • Intervertebral discs
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10
Q

What composes type 3 collagen?

A
  • Newborn skin
  • Aorta
  • Lung
  • Liver
  • Spleen
  • Kidney
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11
Q

What composes type 4 collagen?

A
  • Basement membrane
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12
Q

What is ground substance?

A

Transparent semi-fluid mucopolysaccharide gel

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13
Q

How can ground substance be stained?

A

Carbohydrate Staining

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14
Q

What composes basement membrane?

A
  • Epithelial cells
  • Type 4 collagen
  • glycoproteins
  • proteoglycans
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15
Q

Which staining techniques would you use to stain collagen?

A

Masson trichrome

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16
Q

Which staining techniques would you use to stain Elastic?

A

Verhoeff Van Gieson

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17
Q

Which staining techniques would you use to stain Reticulin?

A
  • Gordon and Sweet’s
  • Silver impregnation
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18
Q

Which staining techniques would you use to stain basement membrane?

A

PAS

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19
Q

Which staining techniques would you use to stain mast cells?

A

Toludine Blue

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20
Q

What is the purpose of Masson Trichrome?

A

demonstrating collagen and fibrotic changes

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21
Q

What is the theory behind Masson Trichrome?

A

Smaller molecules dye the less porous tissue and Larger -> larger pores

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22
Q

What is the idea fixative to use for Masson Trichrome?

A

10% NBF, however it must be post mordant in Bouin’s or Zenker’s

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23
Q

What is the purpose of using Weigert hematoxylin instead of alum hematoxylin in Masson Trichrome?

A

Stronger chemical union

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24
Q

What is PMA/PTA in Masson Trichrome?

A

Mordant differentiator

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25
Which counter stains can be used in Masson Trichrome?
- Blue - Green (best for large amounts)
26
What will nuclei look like following Masson Trichrome?
Blue / Black
27
What will Muscle, cytoplasm and RBC look like following Masson Trichrome?
Red
28
What will Collagen look like following Masson Trichrome?
Green / Blue
29
What is the theory behind Verhoeff van Gieson?
- Hydrogen bonding
30
Which fixative is best for Verhoeff Van Gieson?
- AWFT - NBF - Zenker’s
31
In Verhoeff Van Gieson what is the purpose of ferric chloride?
Mordant and Oxidizer
32
In Verhoeff Van Gieson what is the purpose of hypo?
Remove the excess iodine
33
In Verhoeff Van Gieson what is the purpose of verhoeff’s iodine?
oxidizer and Trapping agent
34
What will nuclei look like following Verhoeff Van Gieson?
Blue / Black
35
What will Elastic fibers look like following Verhoeff Van Gieson?
Blue / Black
36
What will collagen look like following Verhoeff Van Gieson?
Red
37
What will cytoplasm, muscle and look like following Verhoeff Van Gieson?
Yellow
38
What are the three main components of the dye in Gamori’s Aldehyde Fuchsin?
- Rosanaline - Pararosaniline - Basic Fuchsin (Magenta)
39
What is the purpose of Gamori’s Aldehyde Fuchsin?
To stain; - elastic fibers - Mast cell granules - Mucins - Endocrine cells
40
What is the method in which Gamori’s Aldehyde Fuchsin works?
Indirect staining
41
Which counterstains can be used for Gamori’s Aldehyde Fuchsin?
- Light green - Van Gieson
42
What is the result of staining with Gamori’s Aldehyde Fuchsin?
elastic fibers, mast cell granules, mucins, B-cells, pituitary, islet of langerhans ALL ROYAL PURPLE everything else will stain based on the counterstain chosen.
43
What is the preferred fixative of choice for Gamori’s Aldehyde Fuchsin?
NBF
44
What is the purpose of Toludine blue staining method?
Demonstrate metachromatic tissue elements
45
What are metachromatic tissues? and how?
Stain a different colour from the dye solution due to pH, dye concentration, temp and presence of water
46
How are Mast cells different from other metachromatic tissue?
They can undergo alcoholic dehydration due to their stability
47
What will nuclei look like after staining with toludine blue?
Blue
48
What will mast cells look like after staining with toludine blue?
red / purple
49
What is the preferred fixative of choice for toludine blue staining?
NBF
50
What are the idea tissue sections for QC of mast cells?
- GI tract - Skin - Respiratory epithelium
51
What is the purpose of Gordon and Sweet’s silver impregnation?
Aids in differential diagnosis of tumours based on the location of the reticulum
52
In carcinomas what will the reticulum look like?
Reticulum supports a nest of tumors
53
In sarcomas what will reticulum look like?
Mesh like pattern around each tumour
54
In lymphosarcoma what will the reticulum look like?
Each individual cell is surrounded by reticular fiber
55
What is the method by which Gordon and Sweet’s works?
Argyrophilic reactions
56
What is the external reducing agent used in Gordon and Sweet’s?
potassium permanganate
57
What is the purpose of treating sections with iron alum in Gordon and Sweet’s?
Sensitization step
58
How is silver reduced to it’s metallic form in Gordon and Sweet’s?
10% Formalin
59
What will reticulin fibres look like after staining with Gordon and Sweet’s?
Black
60
What will the background look like after staining with Gordon and Sweet’s?
Red
61
What is the counter stain used in Gordon and Sweet’s?
Nuclear Fast red

Histology Exam (8 decks)

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