Connective Tissue Flashcards

(57 cards)

1
Q

origin of connective tissue

functions

A

mesoderm

  1. medium for exchange
  2. fat storage
  3. support
  4. defense and protection
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2
Q

Undifferentiated mesenchymal cell vs Pericyte

origin and site

A

UMC - no origin & in bone marrow as blood cells

peri- around blood capillaries

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3
Q

Undifferentiated mesenchymal cell vs Pericyte
shape?
nucleus?
cytoplasm?

A

UMC - small and branches, pale basophillic cytoplasm, large pale nucleus
Peri- branches w/ long processes , pale basophillic cytoplasm and large oval pale nucleus

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4
Q

Undifferentiated mesenchymal cell vs Pericyte

EM

A

UMC- many free ribosomes but few other organelles

Peri- many free ribosomes but few other organelles and have network of actin and myosin for vasocontriction

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5
Q

Undifferentiated mesenchymal cell functions

A

Can differentiate into:

  1. other types of CT cells
  2. blood cells
  3. smooth muscle cells
  4. endothelial cells
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6
Q

Pericyte functions

A
adult mesenchymal stem cell so in case of injury can differentiate into: 
1. smooth muscle cells
2. endothelial cells
3. fibroblasts
By contraction:
vasoconstriction
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7
Q

origin and site of fibroblast

A

origin : UMC and pericyte

site: in all types of CT proper

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8
Q

Active Fibroblast vs Old Fibroblast (Fibrocyte)
shape?
cytoplasm?
nucleus?

A
active- branches with long thin proccesses 
deep basophillic cytoplasm
large pale nucleus
old- small with few processes
pale basophillic cytoplasm
small darker nucleus
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9
Q

Active Fibroblast vs Old Fibroblast (Fibrocyte)

EM (organelles) nucleus

A

active - well developed rER and golgi
many mitochondria and euchromatic nucleus
old - few organelles, heterochromatin nucleus

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10
Q

functions of active fibroblast

A
  1. synthesis of CT fibers
  2. synthesis of ground substance for matrix
  3. synthesis of growth factors
  4. healing and reapir
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11
Q

functions of old fibroblast (fibrocyte)

A

during injury, it becomes active to heal wounds

slow turnover of extra cellular components fo maintains CT

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12
Q

adipose cell origin and site

A

origin - UMC
site : unilocular - white adipose connective tissue
multilocular - brown adipose connective tissue

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13
Q
Unilocular vs multilocular adipose cell
shape
how is fat stored?
nucleus?
 stain?
A

unilocular - large and oval
fat is stored as one large droplet
peripheral, flat, nucleus
stain : sudan 3 - orange

multilocular - smaller
fat is scattered
nucleus is eccentric and round
pigmented brown color b/c has mitochondria

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14
Q

unilocular fat cells contain

A

dissolved carotenoids

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15
Q

signet ring

A

in unilocular only

when stained with H&E, fat is removed and makes a ring appearance.

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16
Q

Unilocular vs multilocular adipose cell

EM

A

uni- alot of sER
few mitochondria

multi- less sER
many mitochondria rich in cytochrome oxidase

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17
Q

functions of unilocular fat cell

A
  1. synthesis & storage of fat
  2. support organs like kidney
  3. heat insulation
  4. secrete leptin hormone to inhibit food intake
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18
Q

functions of multilocular fat cell

A

thermogenesis which if break down of fat for heat by thermogenin protein in mitochondria

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19
Q

reticular cell vs macrophage

origin and site

A

reticular - UMC
in stroma of glands and organs

macrophage- monocyte
fixed in CT -> histocyte
free in liver -> kupffer cell

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20
Q
reticular cell 
shape
cytoplasm
nucleus
stain
A

small stelate with many long thin proccesses
pale basophillic cytoplasm
pale nucleus
stained with Ag

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21
Q
macrophage
shape
cytoplasm
nucleus
stain
A

large - irregularly shaped b/c pseudopedia
pale basophillic granular cytoplasm bc left overs
essentic, dark, kidney shaped nucleus
vital stains (trypan blue and india ink) are used. you put it on food and macrophage eats it so becomes stained

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22
Q

EM of reticular cell

A

variable amounts of organelle depending on activity

processes are joined by cell junctions

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23
Q

EM of macrophage

A

irregular (pseudopedia)
rich in lysosomes and residual bodies
big golgi and few rER

24
Q

functions of reticular cell

A
  1. secretion of reticular fibers
  2. supportive network
  3. turns phagocytic when stimulated by antigen
  4. antigen presenting cell to activate lymphocyte
25
functions of macrophage
1. phagocytosis 2. multinucleated foreign body giant cell 3. antigen presenting cell 4. product enzymes and cytokines and collegenase 5. destruction of old RBCs
26
origin & site of mast cell
umc Loose CT around blood vessels under epithelium in lung and digestive tube (mucosal mast cell)
27
mast cell shape cytoplasm nucleus stain
large, oval basophillic cytoplasm with basophillic granules central pale nucleus stained metachromatically (purple or red) by toulidine blue
28
EM of mast cell organnelles? nucleus?
``` as protein synthesizing cell well developed rER and golgi many mitochondria euchromatic nucleus electron dense membrane granules ```
29
functions of mast cells
carries receptors for IgE and responsible for: 1. heparin - anticoagulant 2. histamine - vasodilation 3. leukotrienes - bronchal asthma 4. eosinophil chemotactic factor - attracts eosinphil
30
origin and site of plasma cell
b lymphocyte | in lymphoid tissue
31
shape of plasma cell cytoplasm nucleus
large oval deep basophillic cytoplasm with negative golgi image eccentric nucleus with dark heterochromatin and light area of euchromatin - clock face or cart wheel shaped nucleus
32
EM of plasma cell
also as protein synthesizing cell 1. well developed rER and golgi 2. many mitochondria 3. products are secreted so no secretory granules
33
functions of plasma cells
synthesis and secretion of antibodies
34
CT forming cells
fibroblasts, fibrocytes, reticular cells
35
cells responsible for immunity and defence
macrophage, plasma cell, mast cells, leukocytes
36
ground substance is made by | stain
fibroblasts | stained by Ag or metachromatic (toluidine blue)
37
collagen fibers vs electic fibers vs reticular fibers | structure
collagen - collagen protein elastic - elastic protein reticular - collagen 3
38
collagen fibers vs electic fibers vs reticular fibers | synthesis
all - mainly fibroblasts collagen - chrondoblasts (caritlage) , osteaoblasts (bone) elastic - chrondoblasts and smooth muscle cells reticular - smooth muscle cells
39
shape and color collagen fibers vs electic fibers vs reticular fibers
collagen - wavy branching bundles formed of non branching fibers (colorless) elastic - single thin and branching yellow reticular - fiber network branching
40
staining | collagen fibers vs electic fibers vs reticular fibers
collagen - eosin - pink mallory - blue van gieson - red elastic - eosin - pink orcein - brown va geison - yellow reticular silver- brown pas- magenta
41
collagen fibers vs electic fibers vs reticular fibers characters boiling?
collagen - strong and not stretchable flexible but elastic boiled -> gelatin elastic - stretch and recoil resist boiling reticular - loose flexible network
42
functions | collagen fibers vs electic fibers vs reticular fibers
collagen - strength and resist stretching elastic - elasticity reticular - form flexible network
43
vit d deficiency due to symptoms
scurvy due to defective collagen synthesis bleeding gums, loss of teeth, unhealed wounds
44
keloid
local swelling caused by abnormal healing processes leading to increased collagen in scans of skin
45
type 1 collagen main sites and cells of origin
``` bundles CT proper tendon capsule of organs bone origin- fibroblasts and osteoblasts ```
46
type 2 collagen main site and cells of origin
fibrils cartilage origin - chrondroblast
47
type 3 collagen main site and cells of origin
stroma of organs | origin - fibroblast, reticular cell, smooth muscle cell
48
type 4 collagen main site and cells of origin
granular basal lamina associated with epithelium origin- epithelial cells
49
type 5 collagen main site and cells of origin
in dermal epidermal junction of skin | origin- fibroblast
50
loose CT proper
1. loose areolar 2. adipose CT 3. reticular CT 4. Mucoid CT
51
dense CT proper
white fibrous CT | yellow elastic CT
52
loose areolar CT | found where? function?
most common type filled with air cavities, well vascularized, found everywhere except brain exchanges nutrients to and from blod
53
white adipose CT vs brown adipose CT
White- less vascular + carotenoids | Brown - more vascular + cytochrome pigments
54
Mucoid CT which fibers? site function
``` mainly fibroblasts collagen + reticular fibers in hyaluronic acid jelly like in umbulical cord, eye, pulp of teeth for support ```
55
white fibrous CT
fibroblasts very dense and white resistant and less flexible regular - tendon and cornea (regularly arranges collagen bundles and fibroblast in rows) irregular (irregularly arranged collagen with scattered fibroblasts) - dermes, ligaments, capsule of organs, periosteum, perichondrium
56
functions and staining | of regular and irregular white fibrous CT
regular - stretch in one direction, stained blue with mallory irregular - stretch in different reactions, stained blue with mallory
57
yellow elastic tissue | color, site, stain
elastic fibers, yellow in fresh state in aorta and lungs, and ligaments orcein stain - brown