CONTROL OF MICROBIAL GROWTH Flashcards

(54 cards)

1
Q

Enumerate methods of control

A

Physical Agents
Chemical Agents

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2
Q

Enumerate types of physical methods

A

Moist heat
Dry heat
Low temperature
Radiation
Filtration
Dessication

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3
Q

Enumerate: Moist Heat

A

Autoclave
Boiling Water
Pasteurization

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4
Q

Enumerate: dry heat

A

Hot-air Oven
Incineration

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5
Q

Enumerate: Low Temperatures

A

Freezers
Liquid-Nitrogen refrigerators

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6
Q

Enumerate: Radiation

A

Ionizing Radiation
Non-ionizing Radiation

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7
Q

Enumerate Filtration

A

Membrane Filters
High-Efficiency Particulate Air (HEPA) Filters

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8
Q

Factors Influencing the Effective of an Antimicrobial Agent (6)

A

Population size
Population composition
Concentration or intensity of an antimicrobial agent
Duration of exposure
Temperature
Local environment

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9
Q

Used for sterilizing instruments, linens, utensils, treatment trays, media, and other liquids

A

Autoclave

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10
Q

Ineffective against organisms in materials impervious to steam; can’t be used for heat-sensitive articles

A

Autoclave

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11
Q

121 °C

A

Autoclave

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12
Q

15 psi

A

Autoclave

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13
Q

15-30 min

A

Autoclave

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14
Q

100 °C

A

Boiling water

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15
Q

10 min

A

Boiling water

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16
Q

Killing vegetative cells on instruments, and containers

A

Boiling water

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17
Q

Endospores are not killed; can’t be relied upon to sterilize

A

Boiling water

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18
Q

62.8 °C, 30 min or 71.7 °C, 15 min

A

Pasteurization

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19
Q

Killing vegetative cells of disease-causing microorganisms and of many other organisms in milk, fruit juices, and other beverages.

A

Pasteurization

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20
Q

170-180 °C, 1-2 hrs

A

Hot-air oven

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21
Q

Sterilizing material impermeable to or damaged by moisture

A

Hot-air Oven

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22
Q

Destructive to materials that cannot withstand high temperatures

23
Q

Hundreds of °C

24
Q

Sterilization of transfer loops and needles; disposal of carcasses of infected animal; disposal of contaminated objects that cannot be reused

25
Less than 0 °C
Freezers
26
Preservation of foods and other materials
Freezers
27
Mainly microbiostatic instead of microbicidal
Freezers
28
-196 °C
Liquid - Nitrogen refrigerators
29
High cost of liquid nitrogen
Liquid - Nitrogen refrigerators
30
Gamma rays & X-rays
Ionizing Radiation
31
Drive away electrons & split molecules
Ionizing Radiation
32
Able to penetrate packaging and products and sterilize their interiors
Ionizing Radiation
33
Difficult to control due to emission isotopes
Ionizing Radiation
34
Ultraviolet rays
Non-ionizing radiation
35
Excites electrons; damages DNA
Non-ionizing radiation
36
Used to reduce number of microorganisms in air & on surfaces
Non-ionizing radiation
37
Impenetrable to materials
Non-ionizing radiation
38
Used to sterilize fluids by removing microorganisms
Membrane Filters
39
Used to remove or reduce the number of microorganisms in air to prevent their spread in laboratories & hospital environments
High-Efficiency Particulate Air (HEPA) Filters
40
also called freeze drying
lyophilization
41
done by using light, temperature, moisture, lyophilization, and high concentrations
Dessication
42
CHEMICAL METHODS Disinfection of inanimate objects such as instruments, floor and table surfaces, and (with cresols) rectal thermometers
Phenol & Related Compounds
43
CHEMICAL METHODS 0.5-3.0% aqueous solution
Phenol and Related Compounds
44
CHEMICAL METHODS 70 - 90 percent
Alcohols
45
CHEMICAL METHODS Disinfection of skin, delicate surgical instruments, thermometers
Alcohols
46
CHEMICAL METHODS 1.0-2.0%
Iodine
47
CHEMICAL METHODS Disinfection of skin, minor cuts, and abrasions; also used for disinfection of water & swimming pools
Iodine
48
CHEMICAL METHODS 0.5-5.0g available chlorine per liter
Chlorine Compounds
49
CHEMICAL METHODS Disinfection of water, nonmetal surfaces, dairy equipment, restaurant utensils, household items
Chlorine compounds
50
CHEMICAL METHODS 0.1-0.2%
Quarternary Compounds
51
CHEMICAL METHODS Environmental sanitation of surfaces and equipment
Quarternary Compounds
52
CHEMICAL METHODS 1.0%
Mercurial Compounds
53
CHEMICAL METHODS Disinfection of skin, instruments; also used as a preservative in some biological materials
Mercurial Compounds
54
Bacteriostatic agents
Mercurial Compounds