Core Practical: Explain The Effect Of Ph On Enzyme Activity

Question 1

Affect of Ph on enzyme activity practical

Answer 1

• set up a water bath at 35 degrees
• in 3 separate test tubes place 2ml of starch, amylase and buffer solution using 3 separate syringes.
• place a drop of solution onto each well of spotting tile.
• once all solutions are at the same temperature mix starch and amylase solutions and mix using a stirring rod.
• after every 10 seconds use a pipette to transfer the mixture into the wells containing iodine
• if the solution turns black starch is present , if the solution remains yellow/orange the enzymes have digested the starch and the reaction has completed.