COUNTERSTAINS

Question 1

RED cytoplasmic stains

Answer 1

Eosin Y
Eosin B
Phloxine B
Rose Bengal

Question 2

YELLOW cytoplasmic stains

Answer 2

Picric acid
Orange G

Question 3

GREEN cytoplasmic stains

Answer 3

Light green SF
Lissamine green

Question 4

RED nuclear stain

Answer 4

Carmine
Neutral Red
Safranin

Question 5

BLUE nuclear stain

Answer 5

Methylene blue
Toluidine blue
Celestine blue
Hematoxylin

Question 6

simplest connective tissue stain

Answer 6

Van Gieson’s

Question 7

uses acid stain; no color

Answer 7

Masson’s trichrome

Question 8

can also stain hyaline fibrils, amyloid and smooth muscles

Answer 8

Mallory’s Aniline blue

Question 9

for Renal Glomerular basement membraine staining

Answer 9

Azocarmine

Question 10

ELASTIC FIBERS STAINS

Answer 10

1. Weigert’s Elastic tissue stain (dark blue/blue black)
2. Taenzer-Unna Orcein Method (dark brown)
3. Verhoeff’s stain
4. Masson’s trichrome
5. Krajian’s (bright blue)

Question 11

RETICULUM STAINS

Answer 11

1. Silver impregnation technique
2. PAS
3. Bielschowsky
4. Periodic acid leucofuchsin technique
5. Gold method

Question 12

Carbohydrate stain that produce magenta red color

Answer 12

PAS

Question 13

most common method used for the demonstration of glomerular basement membrane of kidney

Answer 13

PAS Technique

Question 14

PAS positive substances

Answer 14

• Carbohydrates
• Phopholipids
• Glycolipids
• Glycoproteins
• Unsaturated lipids
• Mucoproteins

(CAPGGUM)

Question 15

GLYCOGEN STAINS

Answer 15

1.PAS Magenta red
2. Best Carmine - Bright red
3. Langhan’s Iodine - Mahogany brown

Question 16

ACID MUCOPOLYSACCARIDE STAINS

Answer 16

1. Metachromatic stains
2. Colloidal Iron technique
3. Gomori’s aldehude Fuchsin stain
4. Fluorescent acridine orange technique

Question 17

Metachromatic stains

Answer 17

1. Toluidine blue and Azure A
2. Uranyl Nitrate

Question 18

FIBRIN STAINS

Answer 18

1. PAS
2. Gram’s stain
3. MPTAH
4. H & E

Question 19

AMYLOID STAINS

Answer 19

1. Congo red- red/deep pink
2. Gram’s iodine- blue
3. Krajian’s- red
4. Induced fluorescence
5. Metachromatic

Question 20

MUSCLE STAINS

Answer 20

1. MPTAH - black
   2.Lissamine fast red- red

Question 21

BONE STAINS

Answer 21

1. Heidenhain’s s iron hematoxylin with eosin - red
   2.Van Gieson’s Picrofuchsin - bright red
   3.Masson’s trichrome - blue/green

Question 22

Nervous System Stains:

Stains neurons, Axons and neurofibrils

Answer 22

Bielchowsky’s

Question 23

Excellent stain for Astrocytes

Answer 23

Mallory’s Phosphotungstic acid Hematoxylin
(MPTAH)

Question 24

Nervous sytem stain for astrocytes

Answer 24

Cajal’s Gold Sublimate

Question 25

Silver impregnation technique stains

Answer 25

Neuroglia

Question 26

Nervous system stain for myelin sheath

Answer 26

1. Osmic acid 2. Weigert PAL 3. Sudan black

Question 27

Nervous system stains for Nissl bodies/Tigroid substances

Answer 27

1. **H & E** 2. **Thionine** - purple 3. **Toluidine blue** - deep blue 4. **Methylene blue** - deep blue 5. **Cresyl fast violet**

Question 28

Tissue Pigments and Deposit Stains: **For Hemoglobin (black)**

Answer 28

Benzidine

Question 29

Tissue Pigments and Deposit Stains: **For Hemosiderin (deep blue)**

Answer 29

Perl's Prussian Blue

Question 30

Tissue Pigments and Deposit Stains: **For Ferrous ion (blue)**

Answer 30

Turnbull's Blue reaction

Question 31

Tissue Pigments and Deposit Stains: **For bile pigments (green)**

Answer 31

Gmelin's and stein's Iodine test

Question 32

Tissue Pigments and Deposit Stains: **For Hemofuchsin (red)**

Answer 32

Mallory's Fuchsin

Question 33

Tissue Pigments and Deposit Stains: **For Melanin (black)**

Answer 33

Masson Fontana

Question 34

General bacteria stain

Answer 34

Gram's stain

Question 35

for AFB

Answer 35

Ziehl Neelsen's

Question 36

stain for Leishmania, Malaria, trypanosomes, spirochetes and toxoplasmas

Answer 36

Rapid Giemsa

Question 37

for Spirochetes & leprae cells

Answer 37

Levaditi

Question 38

In Levaditi, Spirochetes stains _ on a _ background

Answer 38

BLACK : YELLOWISH BROWN

Question 39

In Warthin Starry, Spirochetes stains _ on a _ background

Answer 39

BLACK : GOLDEN YELLOW

Question 40

For Helicobacter pylori

Answer 40

Warthin Starry