Culture And Culture Media

Question 1

A culture of a staphylococcus aureus only, is considered to be what type of culture?

Answer 1

Pure Culture

Question 2

growth of microorganism

Answer 2

Culture

Question 3

It is composed of more than one species

Answer 3

Mixed culture

Question 4

Composed of several species contained in a separate culture medium

Answer 4

Stock culture

Question 5

TRUE OR FALSE. stock culture’s shelf life should be at least 12 months and an organism should be subcultured twice after thawing to return it to a healthy state.

Answer 5

True 😛

Question 6

What type of culture is used for academic and industrial purposes?

Answer 6

Stock culture

Question 7

It allows the growth of aerobes, anaerobes and facultative anaerobes. It is also prone from contamination

Answer 7

Liquid medium

Question 8

Liquid medium is composed of ___% agar

Answer 8

0% agar

Question 9

What is the indicator for the growth of bacteria in a liquid medium?

Answer 9

Turbidity of the medium

Question 10

A semi-solid medium contains __-__% agar

Answer 10

0.5-1% agar

Question 11

SIM medium is used to identify microorganism’s ______. What does SIM stands for?

Answer 11

Motility; SULFIDE INDOLE MOTILITY

Question 12

It is the most commonly used medium. It contains 2%-3% agar

Answer 12

Solid medium

Question 13

Enumerate 3 liquid medium.

Answer 13

✔️Brain heart infusion
✔️trypticase soy broth
✔️thioglycollate

Question 14

T OR F. Triple sugar iron is a solid media placed in a tube?

Answer 14

True

Question 15

At this range of temperature, agar will melt.

Answer 15

80-90*C

Question 16

What temperature does an agar solidify?

Answer 16

40-50C

Question 17

ture or false. It takes 65-70*C to cool an agar.

Answer 17

False. Bc the cooling temperature of an agar is 55-60 C

Question 18

It is a medium in which all of the components are known to the user and it is used for research purposes

Answer 18

Synthetic/Defined medium

Question 19

The composition is unknown, though it is very useful for the isolation of bacteria.

Answer 19

Non-synthetic/complex medium

Question 20

Used for obligate intracellular bacteria

Answer 20

Tissue culture medium

Question 21

Enumerate the different types of tube medium.

Answer 21

✔️Butt
✔️Butt and slant
✔️Slant
✔️Liquid

Question 22

It is routinely used without added supplement. It also supports he growth of most nonfastidious bacteria.

Answer 22

Simple/general purpose/supportive media

Question 23

A simple/general purpose/supportive media is composed of a ____& ____ extracts.

Answer 23

Meat and soybean extracts

Question 24

it propagates the growth of certain group of bacteria and it is a liquid type.
It is also used as supplement to agar plates

Answer 24

Enrichment media

Question 25

Media with added supplement necessary for the growth of fastidious organisms.

Answer 25

Enriched media/nonselective media

Question 26

Allows visualization of metabolic differences

Answer 26

Differential media

Question 27

Media incorporated with chemicals, dyes or antibiotics to inhibit the growth of other organisms while promoting the growth of the desired organism.

Answer 27

Selective media

Question 28

Selective media for streptococci

Answer 28

Gentamicin blood agar

Question 29

Selective media for hemophilus

Answer 29

Bacitracin chocolate agar

Question 30

Selective media for aeromonas

Answer 30

Blood agar plate with ampicillin

Question 31

Selective media for g+ cocci

Answer 31

Phenyl ehtyl alcohol

Question 32

Selective media for g+ bacteria

Answer 32

Columbia agar with cna

Question 33

Selective media for salmonella ans shiegella spp.

Answer 33

Gram-negative broth

Question 34

Selective media for neisseria gonorrheae

Answer 34

Thayer Martin Agar

Question 35

Isolate bacteria with specific growth requirements

Answer 35

Special media

Question 36

Most commonly performed inoculation technique

Answer 36

Streaking

Question 37

It is used ti differentiate the hemolytic patterns. Also used for fastidious organisms.

Answer 37

Blood Agar

Question 38

It is composed of a mixture of nutrients utilized to observe growth patterns if microorganisms

Answer 38

Culture medium

Question 39

Inhibitory substances for g+ bacteria

Answer 39

Crystal/gentian violet Basic/carbol fuchsin Bile salt

Question 40

Inhibitory substances of G- bacteria

Answer 40

Sodium azide and potassium tellurite

Question 41

Inhibitory substances of swarming bacteria

Answer 41

Alcohol and chloral hydrate

Question 42

What media do you use for the isolation of mycobacteria?

Answer 42

Lowenstein Jensen medium

Question 43

True or false. The specimen on swab is applied by stabbing the swab.

Answer 43

False, because the swab should be applied by rolling the swab onto a small area at the end of the plate, and then streak.

Question 44

What is the manner of reporting of agar-plate colonies?

Answer 44

``` Size Margin or edge Elevation Chromogenesis Optical features ```

Question 45

Manner of reporting griwth on agar slant.

Answer 45

Amount Margin or edge Consistency or mass growth Chromogenesis

Question 46

Manner of reporting of growth in nutrient broth

Answer 46

Amount distribution in broth Odor

Question 47

Manner of reporting of the growth in gelatin stabs

Answer 47

Growth along the line of inoculation | Liquefaction of gelatin