CULTURE & CULTURE MEDIA Flashcards
(121 cards)
1
Q
Pathogens in Gastrointestinal tract
A
SALMONELLA & SHIGELLA
2
Q
lactose fermenters, and non-lactose
fermenters
A
MacConkey
3
Q
hemolytic patterns
(streptococci)
A
BAP (Blood Agar Plate) -
4
Q
Selects the growth of a particular organism at the same time Inhibiting the growth of other organisms.
A
SELECTIVE CULTURE MEDIA
5
Q
Streptococci /Bacitracin
A
Gentamycin blood agar
6
Q
Haemophilus
A
Chocolate agar
7
Q
Aeromonas
A
Blood agar plate w/ ampicillin
8
Q
Gram (+) cocci
A
Phenyl ethyl alcohol
9
Q
gram (+) bacteria
A
Columbia CNA with blood
10
Q
Salmonella spp and Shigella
spp
A
Gram negative (GN) broth
11
Q
Neisseria gonorrhea
A
Thayer Martin Agar (TV)
12
Q
a nutritionally rich formula containing three peptone sources and 5% defibrinated (fibrin has been removed) sheep blood
A
COLUMBIA CNA WITH BLOOD/Columbia agar base
13
Q
refers to the antibiotics colistin (C) and nalidixic acid (NA) that are added to the medium to suppress the growth of most gram-negative organisms while allowing gram-positive
bacteria to grow
A
CNA
14
Q
-an antibiotic that targets the bacterial structure of cell membrane and cell wall
- disrupt the cell membrane of gram negative organisms thus it will not grow
A
Colistin
15
Q
blocks DNA replication of susceptible organism
A
Nalidixic acid
16
Q
used for the cultivation of gastrointestinal pathogens from stool specimens and rectal swabs (for Salmonella and Shigella)
A
GRAM-NEGATIVE BROTH
17
Q
contains sodium citrate and sodium deoxycholate (a bile salt) that inhibit gram-positive organisms and the early multiplication
of gram-negative, non enteric pathogens
A
GRAM-NEGATIVE BROTH
18
Q
Both selective and Enrichment Broth
A
GRAM-NEGATIVE BROTH
19
Q
Primary carbon source of gram-negative broth
A
MANNITOL
20
Q
provides nutrients for common gram-positive
cocci such as enterococci, streptococci, and staphylococci
A
PHENYLETHYL ALCOHOL AGAR (PEA)
21
Q
should not be used in the interpretation of hemolytic reactions
A
PHENYLETHYL ALCOHOL AGAR (PEA)
22
Q
can ONLY be used for hemolytic patterns
A
BAP
23
Q
inhibitory to gram negative
A
CRYSTAL VIOLET
BASIC FUCHSIN
BILE SALT
24
Q
inhibitory to gram positive
A
POTASSIUM TELLURITE
SODIUM AZIDE
25
inhibitory to swarming phenomenon
ALCOHOL
CHLORAL HYDRATE
26
SELECTIVE CULTURE MEDIUM FOR NEISSERIA
Thayer Martin Medium
Martin Lewis Medium
New York City Medium
27
SELECTIVE CULTURE MEDIUM FOR STAPHYLOCOCCUS
Mannitol Salt Agar
Phenyl Ethyl Alcohol Agar
Vogel and Johnson Agar
Chapman Stone Agar
28
SELECTIVE CULTURE MEDIUM FOR GROUP A
STREPTOCOCCUS
Crystal Violet Blood Agar
Polymyxin B, Neomycin acid media
(PNF)
29
SELECTIVE CULTURE MEDIUM FOR GROUP B
STREPTOCOCCUS
Lim, Carrot Broth
Todd and Hewitt Broth with Antibiotics
Granada Agar
30
SELECTIVE CULTURE MEDIUM FOR VIBRIO
Thiosulfate Citrate Bile Salt Sucrose
Monsur Tellurite Taurocholate Gelatin
Agar
31
isolate bacteria with specific growth requirements
SPECIAL MEDIA
32
medium for selective media
Lowenstein-Jensen (L-J) medium
33
TCBS meaning
Thiosulfate Citrate Bile Salt Sucrose
34
specialized medium to isolate Mycobacterium tuberculosis
Lowenstein-Jensen (L-J) medium
35
Selective and differential medium used for isolation and identification of Vibrio cholera
Thiosulfate Citrate Bile Salt Sucrose (TCBS)
36
act as sulfur source
Thiosulfate
37
buffering agent of TCBS
Citrate
38
Inhibit the growth of non-vibrio species
Bile Salts
39
Fermentable sugar that allows for the differentiation of Vibrio species based on their ability to ferment sugar
Sucrose
40
Used when there is an anticipated delay in bringing the specimen into the laboratory
TRANSPORT MEDIUM
41
how many minutes can a transport medium hold a specimen?
30 minutes
42
Transport medium of Streptococcus pyogenes
PIKE'S MEDIA
43
Transport medium of Neisseria
JEMBEC
44
Transport medium of Vibrio cholera
Alkaline Salt Transport Medium
45
Transport medium of Salmonella typhi
Glycerol Saline Transport Media
46
Transport medium of Bordetella
Mishulow's Medium
47
-Used to demonstrate the antibiotic resistance or sensitivity
- an organism to different antibiotics
CULTURE MEDIUM FOR SENSITIVE/SUSCEPTIBILITY TESTING
48
for fastidious organisms
Mueller Hinton Agar (MHA)
49
pH of MHA
7.2 - 7.4
50
Depth of MHA
at least 4 mm
51
Culture medium for sensitivity of Mycobacterium
Middlebrook ZH 10, 7H 11
52
Culture medium for sensitivity of anaerobic bacteria
Wilkin-Chalgren Agar
53
Used to demonstrate biochemical activities of bacteria that is useful in their identification
BIOCHEMICAL MEDIUM
54
special culture medium used to facilitate anaerobic cultivation
THIOGLYCOLATE AND CYSTEINE
55
used to the culture medium to remove (drive off) oxygen to facilitate anaerobic cultivation
BOILING
56
Remove residual oxygen to facilitate anaerobic cultivation
Anaerobic chamber system with a vacuum pump and nitrogen gas
57
convert oxygen to water to facilitate anaerobic cultivation
Gaspak jar containing hydrogen and palladium catalysts
58
Used for small volume to facilitate anaerobic cultivation
plastic bag or pouch containing calcium
carbonate and catalyst
59
Color of Methylene blue in the absence of oxygen
BLUE
60
remove oxygen from the chamber by combining with hydrogen to form water
Palladium pellets
61
diagnostic purposes when infectious disease are involved
CULTURE and CULTURE MEDIA
62
Understand patterns of microbial metabolism in the laboratory
CULTURE and CULTURE MEDIA
63
Develop methods for interrupting their spread and controlling their growth
CULTURE and CULTURE MEDIA
64
Process of cultivating and growing of bacteria in a controlled environment
CULTURE
65
involved providing necessary nutrients, temperature, and other condition in order to support their growth
CULTURE
66
commonly used in industries like biotechnology and pharmaceuticals
BACTERIA
67
where antibiotics came from
BACTERIA
68
provide necessary nutrients like CHO, CHON, salts, and vitamins
CULTURE MEDIA
69
2 MAIN TYPES OF CULTURE MEDIA
SOLID MEDIA & LIQUID MEDIA
70
can be added; not one of the main type
SEMI-SOLID
71
material containing the necessary nutritional and environment requirements for bacterial growth
CULTURE MEDIUM
72
can be liquid, semi-solid or solid preparation utilized to observe growth pattern of microorganism as well as for transport and storage
CULTURE MEDIUM
73
3 TYPES OF CULTURE
PURE CULTURE
MIXED CULTURE
STOCK CULTURE
74
One genus
PURE CULTURE
75
pour the broth of the organism in the agar
pour plate
75
inoculating in agar plate until you can see a single colony
streak plate
76
you’ll get in the mixed culture and if you put it in the selective media, it only allows to grow what it wants to grow
selective media
77
organisms are injected in the animals
animal inoculation
78
more than 2 genus and species
MIXED CULTURE
79
for academic and industrial purposes
STOCK CULTURE
80
3 CLASSIFICATION OF CULTURE MEDIA
According to PHYSICAL STATE or CONSISTENCY
LIQUID FORM
SEMI-SOLID
SOLID
81
Broth, infusion, milk, Brain heart infusion
LIQUID FORM
82
-Allows growth of aerobes, anaerobes and facultative anaerobes
-Nutrients needed are going to dissolve in water
-Bacterial growth indicates by change in appearance
LIQUID FORM
83
if something grows, it will become turbid
(positive)
NUTRIENT BROTH
84
a sulfated polymer made of D-galactose, 3,6 anhydro-L-galactose, and D glucuronic acid and usually derived from red algae
AGAR
85
-Clot like consistency
- used for motility medium, transport medium
SEMI-SOLID FORM
86
doesn’t need oxygen but if you put them in semi-solid form, they usually grow in the bottom
STRICT ANAEROBES
87
they will grow or multiply near the surface
AEROBES
88
% of agar in Semi-Solid Form
0.5% - 1.0% agar
89
% of agar in Solid Form
2-3 %
90
Provides a firm surface on which organisms can form colonies
SOLID FORM
91
Melting point of agar in solid form
80 - 90 degrees celsius
92
Solid Form solidifies at what temp?
40 - 50 degrees celsius
93
2 CLASSIFICATION OF CULTURE MEDIA According to the manner of DISPENSING or DISTRIBUTION
PLATED
TUBED
94
2 CLASSIFICATION OF CULTURE MEDIA According to COMPOSITION
Synthetic or defined medium
Non-synthetic or complex medium
Tissue culture medium
95
All substances are known to the uses
- used for research purposes
SYNTHETIC/DEFINED MEDIUM
96
Composed of some unknown substances
- Very useful for the isolation of bacteria
NON-SYNTHETIC or COMPLEX MEDIUM
97
To isolate obligate intracellular bacteria
TISSUE CULTURE MEDIUM
98
organisms that do not require any special conditions and substances for their growth
Non-fastidious organisms
99
Contains nutrients required to support the growth of wide range/variety of organism
ENRICHED CULTURE MEDIUM
100
For most fastidious bacteria (requires special requirement)
BLOOD AGAR
101
Enriched medium for Haemophilus and Neisseria
CHOCOLATE AGAR
102
2 Enriched culture medium
BLOOD AGAR
CHOCOLATE AGAR
103
most frequently used for primary and selective and differential agars so it contains crystal violet to inhibit the growth of the gram positive bacteria and fungi
CHOCOLATE AGAR
104
also known as Hemin
X FACTOR
105
NAD or NADH
V FACTOR
106
Enumerate the Preferred blood for the preparation of BAP
1st: Sheep
2nd: Horse
3rd: Rabbit
107
contains non-specific inhibitors
Human Blood
108
HUMAN BLOOD CONTAINS
CITRATE
DEXTROSE
109
inhibit growth of (-hemolytic streptococcus)
CITRATE
110
alter types of hemolysis
DEXTROSE
111
Increases the number of pathogens that are outnumbered by non-pathogens
ENRICHMENT CULTURE MEDIUM
112
Extends the lag phase of non-pathogens while decreasing the lag phase of pathogens
ENRICHMENT CULTURE MEDIUM
113
allows or support the growth wide range of organism with additional nutrient
ENRICHED MEDIA
114
with additional nutrient but encourages specific group of organism
ENRICHMENT MEDIA
115
enrichment broth or semisolid media
THIOGLYCOLATE BROTH
116
contains many nutrient factors, including casein, yeast and beef extracts, and vitamins
THIOGLYCOLATE BROTH
117
Distinguishes group of organisms based on cultural characteristics
DIFFERENTIAL CULTURE MEDIUM
118
allows visualization of metabolic differences between group of species of bacteria
DIFFERENTIAL CULTURE MEDIUM
119
contains bile salts and dyes (bromothymol blue and acid fuchsin) to selectively slow the growth of most nonpathogenic gram-negative bacilli found in the gastrointestinal tract
HEKTOEN ENTERIC AGAR (HEA)
120
indicator for the detection of H2 S-producing organisms like Salmonella spp., can be visualized as colonies exhibiting a black precipitate
Ferric ammonium citrate
