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Culture Media Flashcards

(78 cards)

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1
Q

Diff & Iso for Group D Streptococci & Enterococci

A

Bile esculin agar (BEA)

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2
Q

iso & selective for vancomycin resistant enterococci

A

Bile esculin azide agar with vancomycin

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3
Q

Cultivation of nonfastidious microorganisms; determination of hemolytic reactions

A

Blood agar

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4
Q

Iso of Bordetella pertussis and Bordetella parapertussis

A

Bordet-Gengou Agar

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5
Q

Cultivation of fastidious organisms

A

Brain-heart infusion agar or broth

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6
Q

Enrichment of Legionella spp
Growth for Francisella & Nocardia

A

BYCE

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7
Q

Enrichment & Selection of Legionella spp.

A

BCYE + antibiotics

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8
Q

Recovery of Burkholderia cepacia from Cystic fibrosis patients

A

Burkholderia cepacia selective agar

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9
Q

Selec for Campylobacter spp.

A

Campy-blood agar

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10
Q

Selective & holding medium for recovery of Campylobacter spp.
Incubate at 4 degrees celcius for cold enrichment

A

Campylobacter thioglycollate broth

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11
Q

Improved growth of obligate, slow-growing anaerobes

A

CDC anaerobe 5% sheep blood agar

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12
Q

Selective medium for isolation of Campylobacter spp.

A

Cefoperazone, vancomycin, amphotericin (CVA) medium

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13
Q

Selective for Yersinia spp.
Isolation of Aeromonas spp.

A

Cefsulodin-irgasan- novobiocin (CIN) agar

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14
Q

Cultivation of fastidious microorganisms
Haemophilus
Brucella
Pathogenic Neisseria

A

Chocolate agar

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15
Q

Iden of yeasts, MRSA & others

A

Chromogenic media

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16
Q

Selective isolation of G (+) cocci

A

Columbia colistin-nalidixic acid agar (CNA)

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17
Q

Isolation of Corynebacterium diphtheriae

A

Cysteine-tellurite blood agar

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18
Q

Iso & diff of Lactose & Non-lactose fermenting enteric bacilli

A

Eosin-methylene blue agar (EMB) (Levine)

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19
Q

Selective (enrichment) liquid medium for enteric pathogens

A

Gram-Negative broth

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20
Q

Iso,selec,diff of Salmonella & Shigella spp. from other gram-negative enteric bacilli

A

Hektoen Enteric Agar (HE)

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21
Q

Iso & growth of Corynebacterium spp.

A

Loeffler medium

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22
Q

Iso & diff of Lactose & non-lactose fermenting enteric bacilli

A

MacConkey agar

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23
Q

Selec & diff of E. coli O157:H7 in stool specimens

A

MacConkey sorbitol agar

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24
Q

Selective differentiation of Staphylococci

A

Mannitol salt agar

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25
Selective for Neisseria gonorrheae Supports growth of Ureaplasma urealyticum & some Mycoplasma spp.
NYC agar
26
iso & selec of Aerobic gram-positive cocci & bacilli and Anaerobic gram-positive cocci & gram-negative bacilli
Phenylethyl Alcohol agar (PEA)
27
Enrichment & selective for isolation of Bordetella pertussis
Regan Lowe
28
Selective for Salmonella & some Shigella spp
Salmonella-Shigella agar (SS)
29
Nonselective medium for recovery of aerobes & anaerobes Selective for Campylobacter & Helicobacter spp.
Schaedler agar
30
Enrich & isolation of Salmonella spp.
Selenite broth
31
Selective for S. pyo & S. agalactiae
Streptococcal Selective Agar (SSA)
32
Selective for Salmonella & Shigella except Salmonella enterica Typhi
Tetrathionate broth
33
Selective for N. gonorrheae & N. meningitidis
Thayer-Martin agar
34
Supports growth of Francisella and Brucella spp.
Modified Thayer-Martin Agar
35
Supports growth of anaerobes, aerobes, microaerophilic, and fastidious organisms
Thioglycollate broth
36
Selective and diff for Vibrio spp.
Thiosulfate Citrate- Bile Salts agar (TCBS)
37
Selec & enrichment for Streptococcus agalactiae in female genital specimens
Todd-Hewitt broth w/ antibiotics (LIM)
38
Enrichment broth used for subculturing various bacteria from primary agar plates
Trypticase Soy Broth (TSB)
39
Isolation & diff of Salmonella & Shigella spp. from other gram-negative enteric bacilli
Xylose Lysine Deoxycholate Agar (XLD)
40
Yeast extract agar with lysine, xylose, lactose, sucrose, and ferric ammonium citrate; sodium deoxycholate inhibits gram-positive organisms; phenol red as indicator
XLD
41
All-purpose enrichment broth that can support the growth of many fastidious and nonfastidious bacteria
TSB
42
Supplemented with nalidixic acid and gentamicin or colistin for greater selectivity; thioglycollate and agar reduce redox potential
Todd-Hewitt Broth with antibiotics (LIM)
43
Peptone base agar with yeast extract, bile salts, citrate, sucrose, ferric citrate, and sodium thiosulfate; bromthymol blue acts as indicator
TCBS
44
Pancreatic digest of casein, soy broth, and glucose enrich growth of most microorganisms; includes reducing agents thioglycollate, cystine, and sodium sulfite; semisolid medium with a low concentration of agar reducing oxygen diffusion in the medium
Thioglycollate broth
45
Blood agar base enriched with hemoglobin and supplement B; contaminating organisms inhibited by colistin, nystatin, vancomycin, and trimethoprim
TM/MTM agar
46
Peptone-base broth; iodine and potassium iodide, bile salts, and sodium thiosulfate inhibit gram- positive organisms and Enterobacteriaceae
Tetrathionate broth
47
Contains crystal violet, colistin, and trimethoprim-sulfamethoxazole in 5% sheep blood agar base
Streptococcal selective agar (SSA)
48
Peptone and soy protein–base agar with lysed horse blood; vancomycin inhibits gram-positive organisms; polymyxin B and trimethoprim inhibit most gram-negative organisms
Skirrow agar
49
Selective for Campylobacter spp.
Skirrow agar
50
Peptone-base broth; sodium selenite toxic for most Enterobacteriaceae
Selenite agar
51
Peptone and soy protein base agar with yeast extract, dextrose, and buffers; addition of hemin, L-cysteine, and 5% blood enriches for anaerobes
Schaedler agar
52
Peptone base with lactose, ferric citrate, and sodium citrate; neutral red as indicator; inhibition of coliforms by brilliant green and bile salts
SS agar
53
Charcoal agar supplemented with horse blood, cephalexin, and amphotericin B
Regan Lowe
54
Nutrient agar base; phenylethyl alcohol inhibits growth of gram-negative organisms
Phenylethyl Alcohol Agar
55
Peptone agar base with cornstarch, supplemented with yeast dialysate, 3% hemoglobin, and horse plasma; antibiotic supplement includes vancomy- cin (2 μg/mL), colistin (5.5 μg/mL), amphotericin B (1.2 μg/mL), and trimethoprim (3 μg/mL)
NYC
56
Peptone base, mannitol, and phenol red indicator; salt concentration of 7.5% inhibits most bacteria
Mannitol salt agar
57
A modification of MacConkey agar in which lactose has been replaced with D-sorbitol as the primary carbohydrate
MacConkey sorbitol agar
58
Peptone base with lactose; gram-positive organisms inhibited by crystal violet and bile salts; neutral red as indicator
MacConkey agar
59
Peptone-base agar with bile salts, lactose, sucrose, salicin, and ferric ammonium citrate; indicators include bromthymol blue and acid fuchsin
Hektoen enteric (HE) agar
60
Peptone-base broth with glucose and mannitol; sodium citrate and sodium deoxycholate act as inhibitory agents
Gram-negative broth (GN)
61
Peptone base containing lactose; eosin Y and methylene blue as indicators
Eosin methylene blue (EMB) agar (Levine)
62
Infusion agar base with 5% sheep blood; reduction of potassium tellurite by Corynebacterium diphtheriae produces black colonies
Cystine-tellurite blood agar
63
Columbia agar base with 10 mg colistin per liter, 15 mg nalidixic acid per liter, and 5% sheep blood
Columbia colistin- nalidixic acid (CNA) agar
64
Organism-specific nutrient base, selective supple- ments, and chromogenic substrate
Chromogenic media
65
Peptone base, enriched with solution of 2% hemo- globin or IsoVitaleX (BD BBLTM Becton Dickenson, Sparks MD)
Chocolate agar
66
Peptone base with yeast extract, mannitol, and bile salts; supplemented with cefsulodin, irgasan, and novobiocin; neutral red and crystal violet indicators
Cefsulodin-irgasan- novobiocin (CIN) agar
67
Blood-supplemented enrichment medium containing cefoperazone, vancomycin, and amphotericin to inhibit growth of most gram-negative bacteria, gram-positive bacteria, and yeast, respectively
Cefoperazone, vancomy- cin, amphotericin (CVA) medium
68
Tryptic soy broth with phenyl-ethyl alcohol, 5% sheep blood, and added nutrients
CDC* anaerobe 5% sheep blood agar
69
Thioglycollate broth supplemented with increased agar concentration and antibiotics
Campylobacter thioglycollate broth
70
Contains vancomycin (10 mg/L), trimethoprim (5 mg/L), polymyxin B (2500 U/L), amphotericin B (2 mg/L), cephalothin (15 mg/L) in a Brucella agar base with sheep blood
Campy-blood agar
71
Bile salts, gentamicin, ticarcillin, polymyxin B, peptone, yeast extract
Burkholderia cepacia selective agar
72
BCYE supplemented with polymyxin B, vancomycin, and ansamycin to inhibit gram-negative bacteria, gram-positive bacteria, and yeast, respectively
Buffered charcoal–yeast extract (BCYE) agar with antibiotics
73
Yeast extract, agar, charcoal, and salts supplemented with L-cysteine HCl, ferric pyrophosphate, ACES buffer, and alpha-ketoglutarate
Buffered charcoal–yeast extract agar (BCYE)
74
Dextrose, pork brain, and heart dehydrated infusions
Brain-heart infusion agar or broth
75
Potato-glycerol–based medium enriched with 15%- 20% defibrinated blood; contaminants inhibited by methicillin (final concentration of 2.5 μm/mL)
Bordet-Gengou agar
76
Trypticase soy agar, Brucella agar, or beef heart infusion with 5% sheep blood
Blood agar (BA)
77
Contains azide to inhibit gram-negative bacteria, vancomycin to select for resistant gram-positive bacteria, and bile esculin to differentiate entero- cocci from other vancomycin-resistant bacteria that may grow
Bile esculin azide agar with vancomycin
78
Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria.
Bile esculin agar (BEA)

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