CULTURE MEDIA

Question 1

What are the primary culture media used in mycology?

Answer 1

SABOURAUD DEXTROSE AGAR
BRAIN HEART INFUSION
SABOURAUD HEART INFUSION
INHIBITORY MOLD AGAR
POTATO DEXTROSE AGAR
POTATO FLAKE AGAR
V8 AGAR

Question 2

What are the selective culture media used in mycology?

Answer 2

Mycosel/Mycobiotic Agar
Corn meal Agar w/ Tween 80
Czapek Agar
Niger Agar/Birdseed Agar
Cotton seed Agar

Question 3

What are the selective/differential media?

Answer 3

Rice Extract Agar
Chrom Agar

Question 4

What are the differential Media?

Answer 4

Trichophyton Agar
Urea Agar

Question 5

For the Recovery of Pathogenic Fungi

Answer 5

SDA

Question 6

what are the three organisms that are usually isolated in SDA without antibiotics

Answer 6

Aspergillus
C. neoformans
Candida

Question 7

Primary recovery of saprophytic and dimorphic fungi, particularly fastidious strains

Answer 7

Brain Heart Infusion Agar

Question 8

SDA+BHI=
(better combined)
Perfect for dimorphic fungi

Answer 8

SABOURAUDS HEART INFUSION AGAR

Question 9

Primary Recovery of dimorphic pathogenic fungi

Answer 9

Inhibitory mold agar

Question 10

what are the two organisms that will not be recovered in IMA

Answer 10

Saprophytic fungi & Dermatophytes

Question 11

Stimulates sporulation and pigmentation

Answer 11

Potato Dextrose Agar

Question 12

Lowers the pH by 3.5 in PDA

Answer 12

10% Tartaric Acid

Question 13

Primary recovery of Saprophytic and Dimorphic Fungi (Fastidious and slow growing)

Answer 13

Potato Flake Agar

Question 14

Recommended for the cultivation of yeasts and molds

Answer 14

V8 agar

Question 15

4 compositions of V8 agar

Answer 15

Yeast extract
glucose
v8 juice
L-asparagine

Question 16

A highly selective media for the isolation of pathogenic fungi for non-sterile materials also used for the recovery of dermatophytes

Answer 16

Mycosel Agar/Mycobiotic Agar

Question 17

what are the two antibiotics used in mycosel agar

Answer 17

Cycloheximide and Chloramphenicol

Question 18

this is used for the inducement of Chlamydospore formation of Candida albicans

Answer 18

Cornmeal Agar w/ tween 80

Question 19

two purposes of tween 80

Answer 19

growth of candida
reduce the solid portion of the cornmeal to grow the pseudohyphae

Question 20

for the routine cultivation fungi especially:
aspergillus
penicillium
non-sporulating molds

Answer 20

CZAPEK AGAR

Question 21

CZAPEK AGAR IS USED FOR THE ROUTINE CULTIVATION OF?

Answer 21

ASPERGILLUS
PENICILLIUM
NON-SPORULATING MOLDS

Question 22

a selective media used for the Identification of C. Neoformans

Answer 22

NIGER SEED/BIRDSEED AGAR

Question 23

NIGER SEED/BIRDSEED AGAR detects the enzyme?

Answer 23

Phenoloxidase

Question 24

Bird Seed Agar contains:

Answer 24

Caffeic Acid
creatinine (Enhance Melanin)
Glucose
Chloramphenicol

Question 25

Caffeic Acid + Phenoloxidase =

Answer 25

Melanin

Question 26

A selective media used for the conversion of Blastomyces dermatitidis from mold to yeast

Answer 26

Cottonseed Conversion Agar

Question 27

for the differentiation of yeasts by means of their typical chlamydospores and on the basis of micromorphological Criteria useful in growing Candida spp.

Answer 27

Rice Extract Agar

Question 28

for the differentiation of clinically-significant Candida Species

Answer 28

CHROM agar media

Question 29

Give the color of the following in CHROM agar: C. albicans C. tropicalis C. glabrata C. krusei

Answer 29

aqua blue (C. albicans) dark blue (C. tropicalis) smooth dark pink (C. glabrata) fluffy pink (C. krusei)

Question 30

It is a differential media but also selective because it only grows Trychophyton species

Answer 30

Trichophyton Agars

Question 31

What are the 4 trichophyton agars and their composition

Answer 31

1: vitamin free 2: Inositol 3: Inositol and Thiamine 4: Thiamine

Question 32

Demonstrates the enzyme UREASE

Answer 32

UREA AGAR

Question 33

UREA AGAR DETECTS?

Answer 33

C. neoformans T. mentagrophytes vs T. rubrum Trichosporon spp.

Question 34

Urease breaks down urea to:

Answer 34

Ammonia, H2O, CO2