Culture media Flashcards by Maan Roño

Cary and Blair medium

Maintains viability of pathogens from source to laboratory

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Amie’s medium

Maintains viability of pathogens from source to laboratory

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Alkaline peptone water

To increase number of pathogenic vibrio in the specimen

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Gram negative broth

To increase number of pathogens in stools

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Selenite F broth

For enrichment and isolation of Salmonella spp.

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Tetrathionate broth

For isolation of Salmonella and Shigella spp. from stools

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Todd-Hewitt broth supplemented with antibiotics

For enrichment and isolation of Streptococcus agalactiae

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Anaerobic blood agar

For isolation of all anaerobes

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Anaerobic phenylethylalcohol (PEA) agar

For obligate Gram (+) and Gram (-) anaerobes

It inhibits growth of enteric bacilli & swarming of Proteus

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Anaerobic kanamycin vancomycin blood agar

Gram (-) anaerobes esp. Bacteroides, Prevotella & some Fusobacterium

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Anaerobic paroromycin vancomycin laked blood agar

Gram (‐) anaerobes esp. Bacteroides, Prevotella & some Fusobacterium
Detection of pigment production of Prevotella

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Brain Heart Infusion (BHI) broth or agar

Basic medium for the isolation of microorganisms & can be used for preparing pure culture for antibiotic susceptibility test

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Trypticase soy broth or agar

Supports growth of many nonfastidious and fastidious bacteria. It can be used as a base in preparing blood agar

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Blood agar

Enriched with unheated blood

Differentiate organisms thru hemolytic patterns

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Chocolate agar

Enriched with heated blood

For the cultivation of most organisms esp. Haemophilus spp. and Neisseria spp.

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Dextrose ascitic fluid agar

To isolate organisms in CSF

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Cystine trypticase agar (CTA)

For identification of Neisseria spp. Based on their ability to utilize carbohydrate

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Lysine iron agar (LIA)

For the identification of Gram (‐) enteric bacilli based on their ability to decarboxylate or deaminate lysine and to produce hydrogen sulfide or not

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Litmus milk medium

For the isolation of Clostridium perfringens

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Moeller’s broth

For the identification of Gram (-) enteric bacilli based on their ability to produce LDC/ ODC/ ADH

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Oxidation-fermentation (O-F) medium

For the differentiation of organisms based on their ability to oxidize or ferment carbohydrates

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Tryptone broth

For the identification of Gram (‐) enteric bacilli based on their ability to produce indole

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Triple sugar iron (TSI) agar

For the identification of Gram (-) enteric bacilli based on their ability to ferment glucose, lactose and sucrose, produce gas and hydrogen sulfide

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Methyl red voges proskauer (MRVP) broth / Peptone glucose broth

For the identification of Gram (-) enteric bacilli based on their ability to ferment glucose via mixed acid fermentation or butylene glycol pathway

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Christensen urea agar / Stuart urea broth

For the identification of Gram (‐) enteric bacilli based on their ability to produce enzyme urease

Phenylalanine deaminase agar

For the identification of Gram (-) enteric bacilli based on their ability to produce enzyme deaminase

Simmon citrate agar

For the identification of Gram (-) enteric bacilli based on their ability to utilize citrate as source of carbon

PYR agar

To diff. beta hemolytic streptococci and enterococci based on their ability to produce enzyme pyrrolidonnylaryamidase or pyroglutamylaminopeptidase

Acetate slant

For the identification of organisms which utilize acetate as a source of carbon like E. coli

Acetamide slant

For the identification of organisms which utilize acetamide as a source of carbon like Pseudomonas aeruginosa

DNA agar

To identify Staphylococcus aureus based on its ability to produce DNASE enzyme

Egg yolk agar (EYA)

To identify Clostridium perfringens based on its ability to produce lecithinase

12% gelatin medium

For the identification of organisms which liquefy or hydrolyze gelatin

Mueller-Hinton agar/broth

For antibiotic susceptibility test of most bacteria

McCoy cells

For the isolation of Chlamydia

HeLa cell line

For the isolation of Rickettsiae

Embryonated egg

For the isolation of Rickettsiae

Schaedler agar

For the recovery of aerobes and anaerobes

Seller's agar

For diff. nonfermentative like Pseudomonas, Acinetobacter & Alkaligenes

Salt (NaCl) broth

For the identification of organisms based on their ability to grow or not to grow in the presence of 6.5% NaCl

Scott's modified Castañeda and thioglycollate broth

For blood culture

Thioglycollate broth

For the recovery of aerobes, anaerobes, microaerophilic and fastidious microorganisms

Food pad of mice and armadillo

For the isolation and identification of Mycobacterium leprae

Bile esculin medium (BEM)

Group D Streptococcus & Enterococcus

Bismuth sulfite agar (BSA) or Wilson Blair agar

Salmonella typhi

Brucella blood agar

Brucella spp.

Biphasic media of Brucella Broth & Agar in Castañeda bottle or septi- chek

Brucella spp.

Blood cystine glucose agar with thiamine

Francisella tularensis

Buffered charcoal yeast extract agar (BCYE)

Legionella pneumophila

Cefsulodin irgasan novobiocin

Yersinia enterocolitica

Campy blood agar

Campylobacter spp.

Campylobacter thioglycollate broth

Campylobacter spp.

Cefoperazone, vancomycin, amphotericin medium

Campylobacter spp.

Skirrow's medium

Campylobacter spp.

Chapman-Stone agar

Staphylococci

Columbia colistin nalidixic agar (CNA)

Staphylococci & Streptococci from mixed specimen

Cooked meat or chopped meat medium

Anaerobes

Lombard-Dowell agar

Anaerobes

Cycloserine cefoxitin fructose agar (CCFA)

Clostridium difficile

Cystine lactose electrolyte deficiend (CLED) agar

Bacteria in urine

Cystine tellurite blood agar

Corynebacterium diphtheriae

Loeffler's coagulated serum slant

Corynebacterium diphtheriae

Tinsdale agar

Corynebacterium diphtheriae

Pai's agar

Corynebacterium diphtheriae

McLeod's agar

Corynebacterium diphtheriae

Clostrisel agar

Clostridium perfringens

Dieudonne's medium

Vibrio spp.

Thiosulfate citrate bile salts sucrose (TCBS) agar

Vibrio spp.

E agar

Mycoplasma & Ureaplasma spp.

Edward Hayflick agar

Mycoplasma & Ureaplasma spp.

Eosin methylene blue (EMB) agar

Gram (‐) bacilli | Diff. lactose from non-lactose fermenting gram (-) enteric bacilli

Hektoen enteric agar (HEA)

Gram (‐) bacilli | Diff. lactose from non-lactose fermenting gram (-) enteric bacilli

MacConkey agar

Gram (‐) bacilli | Diff. lactose from non-lactose fermenting gram (-) enteric bacilli

Ellinghausen, McCullough, Johnson & Harris (EMJH) medium

Leptospira interrogans

Fletcher's semisolid medium

Leptospira interrogans

Fetal bovine serum agar with vancomycin

Haemophilus ducreyi

Human blood bilayer tween (HBT) agar

Gardnerella vaginalis

Modified Kelly's medium or Barbour-Stoenner-Kelly (BSK-II)

Borrelia spp.

Lowenstein Jensen (LJ) agar

Mycobacterium spp.

Lowenstein Jensen (LJ) agar with antibiotics

Mycobacterium spp.

Middlebrook 7H9 (liquid)/7H10/7H11 (solid)

Mycobacterium spp.

Mitchison agar

Mycobacterium spp.

Dorset egg medium

Mycobacterium spp.

Petragnani medium

Mycobacterium spp.

American thoracic society (ATS) medium

Mycobacterium spp.

Mannitol salt agar (MSA)

Mannitol and non‐mannitol fermenting Staphylococci

Martin Lewis agar

Neisseria spp.

Modified Thayer Martin medium

Neisseria spp.

McBride medium

Listeria monocytogenes

New York City (NYC) agar

Pathogenic spp. of Neisseria and also Mycoplasma hominis & Ureaplasma urealyticum

Wallenstein medium

Non‐tuberculous mycobacteria

Phenylethanol agar (PEA)

Gram (+) cocci and anaerobic gram (‐) bacilli

Regan Lowe agar

Bordetella pertussis

Border Gengou agar or potato blood glycerol agar

Bordetella pertussis

Salmonella-Shigella agar (SSA)

Salmonella and Shigella

Streptococcal selective agar

Streptococcus pyogenes and Streptococcus agalactiae

Thayer Martin agar

Pathogenic spp. of Neisseria

Transgrow medium

Neisseria gonorrhoeae

Cetrimide agar slant

Pseudomonas aeruginosa

Xylose lysine desoxycholate (XLD) agar

Gram (‐) enteric bacilli and diff. of Salmonella and Shigella spp.

Bacteroides bile esculin (BBE) medium

Bacteroides spp. and diff. bile resistant from bile sensitive Bacteroides spp.

Shepard's A7-B agar

Mycoplasma spp.

Stuart's medium

Maintains viability of pathogen from source to the laboratory

Culture media Flashcards

(103 cards)